An improved assay for DNA ligase reveals temperature-sensitive activity in cdc9 mutants of Saccharomyces cerevisiae.

Molecular & general genetics : MGG ·Vol. 200 ·No. 3 ·1985-00-00 ·Pages 458-62

Barker DG, Johnson AL, Johnston LH

Abstract

A sensitive and quantitative assay for DNA ligase has been developed which is suitable for the analysis of crude cell extracts of yeast. The assay is sufficiently sensitive to detect the low levels of DNA ligase activity remaining in cdc9 mutants of Saccharomyces cerevisiae. Indeed, we have been able to show that this residual activity is temperature-sensitive, thus establishing finally that CDC9 is the structural gene for DNA ligase.

MeSH Terms

Base Sequence DNA Ligases/genetics,metabolism Kinetics Mutation Polynucleotide Ligases/metabolism Saccharomyces cerevisiae/enzymology,genetics Temperature Templates, Genetic

Chemicals

DNA Ligases Polynucleotide Ligases

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Barker D G

Johnson A L

Johnston L H

References (11)

11 references, click to expand

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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00425731

Published

1985-00-00

Pages

458-62

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00425731

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An improved assay for DNA ligase reveals temperature-sensitive activity in cdc9 mutants of Saccharomyces cerevisiae.

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