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PMID: 3900642 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Isolation of genes from Candida albicans by complementation in Saccharomyces cerevisiae.

Molecular & general genetics : MGG ·Vol. 200 ·No. 3 ·1985-00-00 ·Pages 500-2

Rosenbluh A, Mevarech M, Koltin Y, Gorman JA

Abstract

A genomic library of the asexual pathogenic yeast Candida albicans was constructed in the S. cerevisiae vector YEp13. The library contains a representation of the entire genome with a probability of 99%. The expression of the genes of C. albicans in S. cerevisiae was examined and two mutations his3-1 and trp1-289 of S. cerevisiae were complemented by the cloned genes of C. albicans. The hybridization data indicates that the plasmids complementing the mutations of S. cerevisiae contain sequences from C. albicans.

MeSH Terms

Candida albicans/genetics Escherichia coli/genetics Genes, Viral Genetic Complementation Test Genetic Vectors Mutation Plasmids Saccharomyces cerevisiae/genetics

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Rosenbluh A

Mevarech M

Koltin Y

Gorman J A

References (17)

17 references, click to expand

Heterozygosity and segregation in Candida albicans.
Mol Gen Genet. 1980;180(1):107-13 PMID: 7003306
Parasexual genetic analysis of Candida albicans by spheroplast fusion.
J Bacteriol. 1981 Jun;146(3):833-40 PMID: 7016832
Isolation and sequence of the gene for actin in Saccharomyces cerevisiae.
Proc Natl Acad Sci U S A. 1980 Jul;77(7):3912-6 PMID: 7001447
The primary structure of the alcohol dehydrogenase gene from the fission yeast Schizosaccharomyces pombe.
J Biol Chem. 1983 Jan 10;258(1):143-9 PMID: 6294096
Genetic analysis of Candida albicans: identification of different isoleucine-valine, methionine, and arginine alleles by complementation.
J Bacteriol. 1982 Sep;151(3):1247-52 PMID: 7050083
Recombination analysis of naturally diploid Candida albicans.
J Bacteriol. 1982 Dec;152(3):969-75 PMID: 6754707
Isolation of the beta-tubulin gene from yeast and demonstration of its essential function in vivo.
Cell. 1983 May;33(1):211-9 PMID: 6380751
UV-induced mitotic co-segregation of genetic markers in Candida albicans: Evidence for linkage.
Curr Genet. 1983 Jun;7(3):167-73 PMID: 24173274
Genetic analysis of red, adenine-requiring mutants of Candida albicans.
J Bacteriol. 1983 Dec;156(3):1066-77 PMID: 6358187
Molecular genetics of yeast.
Annu Rev Biochem. 1980;49:845-76 PMID: 6996573
Natural heterozygosity in Candida albicans.
J Bacteriol. 1981 Feb;145(2):896-903 PMID: 6780534
Studies on the pink, adenine-deficient strains of Candida albicans. I. Cultural and morphological characteristics.
Sabouraudia. 1970 May;8(1):48-59 PMID: 5427154
A genetic analysis of Candida albicans: isolation of a wide variety of auxotrophs and demonstration of linkage and complementation.
Genetics. 1983 Jun;104(2):241-55 PMID: 6345266
Isolation of a gene from Drosophila by complementation in yeast.
Nature. 1981 Jan 1;289(5793):33-7 PMID: 6256646
Transformation in yeast: development of a hybrid cloning vector and isolation of the CAN1 gene.
Gene. 1979 Dec;8(1):121-33 PMID: 395029
DNA content, kinetic complexity, and the ploidy question in Candida albicans.
Mol Cell Biol. 1982 Jul;2(7):853-62 PMID: 6765567
Hybridization of Candida albicans through fusion of protoplasts.
Arch Microbiol. 1981 Mar;129(1):1-8 PMID: 7013727

Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00425739

Published

1985-00-00

Pages

500-2

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00425739

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Isolation of genes from Candida albicans by complementation in Saccharomyces cerevisiae.

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