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PMID: 3901002 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Isolation of the human insulin-like growth factor genes: insulin-like growth factor II and insulin genes are contiguous.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 19 ·1985-10-00 ·Pages 6450-4

Bell GI, Gerhard DS, Fong NM, Sanchez-Pescador R, Rall LB

Abstract

Overlapping recombinant clones that encompass the insulin-like growth factor (IGF) I and II genes have been isolated from a human genomic DNA library. Each gene is present once per haploid genome; the IGF-I gene spans greater than 35 kilobase pairs (kbp) and the IGF-II gene is at least 15 kbp. The exon-intron organization of these genes is similar, each having four exons, which is one more than the related insulin gene. Comparison of the restriction endonuclease cleavage maps of the IGF-II and insulin genes, including their flanking regions and hybridization with an IGF-II cDNA probe, revealed that they are adjacent to one another. The IGF-II and insulin genes have the same polarity and are separated by 12.6 kbp of intergenic DNA that includes a dispersed middle repetitive Alu sequence. The order of the genes is 5'-insulin-IGF-II-3'.

MeSH Terms
Base Sequence Cloning, Molecular DNA, Recombinant/analysis Genes Genetic Linkage Humans Insulin/genetics Insulin-Like Growth Factor I/genetics Insulin-Like Growth Factor II/genetics RNA, Messenger/analysis Recombinant Proteins/genetics Somatomedins/genetics
Chemicals
DNA, Recombinant Insulin RNA, Messenger Recombinant Proteins Somatomedins Insulin-Like Growth Factor I Insulin-Like Growth Factor II
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Bell G I
Gerhard D S
Fong N M
Sanchez-Pescador R
Rall L B
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46 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.19.6450
Published
1985-10-00
Pages
6450-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC390734
Subset
IM
Grants
NCI NIH HHS · CA 17575 · United States
NIGMS NIH HHS · GM 27882 · United States
External Links
PubMed source DOI: 10.1073/pnas.82.19.6450 PMC Full Text View journal details
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