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PMID: 3901700 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Free lung cell phagocytosis and lysosomal enzyme activity after inhalation of lipopolysaccharide in guinea-pigs.

Agents and actions ·Vol. 16 ·No. 5 ·1985-07-00 ·Pages 353-8

Rylander R, Fogelmark B, Sjöstrand M

Abstract

Free lung cells were studied in guinea-pigs different times after an inhalation exposure to bacterial endotoxin yielding a dose of about 1 microgram/animal. After exposure the number of alveolar macrophages (AM) and neutrophils was increased. The phagocytosis capacity of the lung was increased, mainly due to the influx of neutrophils. The activity of N-acetyl-beta-D-glucosaminidase from exposed animals was decreased in AM cell cultures and increased in lavage fluid 2 hours and later after exposure. Cathepsin D activity was also decreased in AM cell cultures but did not change in lavage fluid. Lactate dehydrogenase was increased in lung lavage fluid. The results show that LPS inhalation activates AM and that increased amounts of lysosomal enzymes are present in the airways.

MeSH Terms

Acetylglucosaminidase/metabolism Animals Cathepsin D/metabolism Escherichia coli Female Guinea Pigs Kinetics L-Lactate Dehydrogenase/metabolism Lipopolysaccharides/pharmacology Lung/cytology Lysosomes/enzymology Macrophages/physiology Male Neutrophils/physiology Phagocytosis Therapeutic Irrigation

Chemicals

Lipopolysaccharides L-Lactate Dehydrogenase Acetylglucosaminidase Cathepsin D

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Rylander R

Fogelmark B

Sjöstrand M

References (13)

13 references, click to expand

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Article Info

Journal

Agents and actions

Abbr.

Agents Actions

ISSN

0065-4299

DOI

10.1007/BF01982872

Published

1985-07-00

Pages

353-8

Language

English

Region

Switzerland

NLM ID

0213341

Subset

External Links

PubMed source DOI: 10.1007/BF01982872

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Free lung cell phagocytosis and lysosomal enzyme activity after inhalation of lipopolysaccharide in guinea-pigs.

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