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PMID: 390306 Published · ppublish English Comparative Study Journal Article

Inheritance of multiple drug resistance in Saccharomyces cerevisiae: linkage to leu1 and analyses of 2 micron DNA in partial revertants.

Molecular & general genetics : MGG ·Vol. 175 ·No. 1 ·1979-08-00 ·Pages 45-52

Saunders GW, Rank GH, Kustermann-Kuhn B, Hollenberg CP

Abstract

The inheritance and phenotype of multiple drug resistance in independent multiple drug resistant mutants, two isolated in this laboratory (GR359 and 2-20), and two (DRI 9/T7 and DRI 9/T8) reported by Guerineau et al. (Biochem. Biophys. Res. Commun. 61,462), was investigated. Comparison of resistance to 12 selected drugs showed that the resistance phenotypes of all mutants were similar, although some differences in levels of resistance of each mutant was observed with certain drugs. Mapping of the resistance loci in GR359 and 2-20 revealed tight linkage of both resistance genes to the centromere linked gene leul. 2 micron DNA was analysed by hybridization of 2 micron RNA to EcoRI fragments of a total DNA extract. Eight partial revertants of 2-20, which had been chosen as having a phenotype similar to the 2 micron DNA deficient [cir degrees] isolate DRI 9/T7, revealed the presence of 2 micron DNA. The lack of detectable 2 micron DNA in DRI 9/T7 was confirmed.

MeSH Terms
DNA, Fungal/analysis Drug Resistance, Microbial Genes Genetic Linkage Mutation Nucleic Acid Hybridization Phenotype RNA, Fungal/genetics Saccharomyces cerevisiae/genetics
Chemicals
DNA, Fungal RNA, Fungal
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Saunders G W
Rank G H
Kustermann-Kuhn B
Hollenberg C P
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17 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00267854
Published
1979-08-00
Pages
45-52
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00267854
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