Purification and crystallization of fimbrial hemagglutinin from Bordetella pertussis.

Infection and immunity ·Vol. 25 ·No. 1 ·1979-07-00 ·Pages 460-2

Arai H, Munoz JJ

Abstract

A procedure to purify and crystallize fimbrial hemagglutinin from Bordetella pertussis is described. Redissolved crystals had the same specific activity as the original, purified solution of fimbrial hemagglutinin. About 97% of the weight of washed crystals was accounted for by amino acids.

MeSH Terms

Agglutinins/isolation & purification Antibodies, Bacterial/analysis,isolation & purification Bordetella pertussis/immunology,ultrastructure Crystallization Fimbriae, Bacterial/immunology Hemagglutinins/analysis,isolation & purification

Chemicals

Agglutinins Antibodies, Bacterial Hemagglutinins

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Arai H

Munoz J J

References (7)

7 references, click to expand

Production, extraction and purification of haemagglutinin of Haemophilus pertussis.
J Gen Microbiol. 1952 Nov;7(3-4):201-10 PMID: 13022907
Studies on the direct hemagglutinin in fluid culture supernatant of H. pertussis.
Kitasato Arch Exp Med. 1953 Nov;26(2-3):151-64 PMID: 14382303
Studies on the hemagglutinin of Hemophilus pertussis.
J Immunol. 1950 Dec;65(6):627-32 PMID: 14803714
Adsorption of protective antigen of Hemophilus pertussis on human red cell stromata.
Proc Soc Exp Biol Med. 1950 Dec;75(3):704-5 PMID: 14808372
The haemagglutinin of Haemophilus pertussis. IV. Reactions and antibody response in babies and children following the administration of haemagglutinin absorbed on aluminum phosphate.
Aust J Exp Biol Med Sci. 1951 Nov;29(6):425-32 PMID: 14904319
Separation and characterization of two distinct hemagglutinins contained in purified leukocytosis-promoting factor from Bordetella pertussis.
Biochim Biophys Acta. 1976 Oct 22;444(3):765-82 PMID: 186106
Leukocytosis-promoting factor of Bordetella pertussis. 3. Its identity with protective antigen.
Infect Immun. 1974 May;9(5):801-10 PMID: 4363229

Article Info

Journal

Infection and immunity

Abbr.

Infect Immun

ISSN

0019-9567

DOI

10.1128/iai.25.1.460-462.1979

Published

1979-07-00

Pages

460-2

Language

English

Region

United States

NLM ID

0246127

PMCID

PMC414472

Subset

External Links

PubMed source DOI: 10.1128/iai.25.1.460-462.1979 PMC Full Text View journal details

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Purification and crystallization of fimbrial hemagglutinin from Bordetella pertussis.

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