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PMID: 3904736 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Fluorescence studies on the nucleotide- and Ca2+-binding domains of molluscan myosin.

The Biochemical journal ·Vol. 231 ·No. 1 ·1985-10-01 ·Pages 31-8

Wells C, Warriner KE, Bagshaw CR

Abstract

The effects of nucleotides and Ca2+ on the intrinsic tryptophan fluorescence of molluscan myosin and its proteolytic fragments were studied. By using these proteins from the scallop, Pecten maximus, the existence of two distinct tryptophan-containing domains was established, which respond independently to ATP and Ca2+-specific binding. The latter is located in the 'neck' region of the myosin, which constitutes the regulatory domain. Subfragment 1, lacking the regulatory domain, responded only to ATP binding. On the other hand a tryptic fragment comprising the regulatory domain responded only to Ca2+ binding. Subfragment 1, containing the regulatory domain, responded to both ATP and Ca2+, but its ATPase activity was Ca2+-insensitive. By contrast, the ATPase activity of HMM was Ca2+-sensitive. Increasing the ionic strength had a detrimental effect on Ca2+-sensitivity, and fluorescence studies on solubilized myosin were therefore of limited value. Myosin and its fragments from other molluscan species which were investigated produced similar changes to those of Pectan maximus.

MeSH Terms

Adenosine Diphosphate/metabolism Adenosine Triphosphate/metabolism Animals Calcium/metabolism Electrophoresis, Polyacrylamide Gel Mollusca/metabolism Myosin Subfragments/metabolism Myosins/metabolism Peptide Fragments/analysis Protein Binding Spectrometry, Fluorescence Tryptophan/analysis

Chemicals

Myosin Subfragments Peptide Fragments Adenosine Diphosphate Tryptophan Adenosine Triphosphate Myosins Calcium

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Wells C

Warriner K E

Bagshaw C R

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25 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj2310031

Published

1985-10-01

Pages

31-8

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1152699

Subset

External Links

PubMed source DOI: 10.1042/bj2310031 PMC Full Text View journal details

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Fluorescence studies on the nucleotide- and Ca2+-binding domains of molluscan myosin.

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