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PMID: 3905825 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Human endothelial cells are chemotactic to endothelial cell growth factor and heparin.

The Journal of cell biology ·Vol. 101 ·No. 6 ·1985-12-00 ·Pages 2330-4

Terranova VP, DiFlorio R, Lyall RM, Hic S, Friesel R, Maciag T

Abstract

The response of human endothelial cell migration to various extracellular matrix components and growth factors has been assessed. Human endothelial cells demonstrate increased chemotaxis and chemokinesis when placed in a modified Boyden chamber with endothelial cell growth factor (ECGF) used at a concentration of 10(-9) M. Anti-ECGF antibody inhibits the chemotactic response. Heparin (10(-8) to 10(-10) M) was also chemotactic and was shown to potentiate the chemotactic activity of ECGF. Although laminin, fibronectin, the polypeptide (epidermal, fibroblast, and nerve) growth factors, and collagen types I, II, III, IV, and V demonstrate a chemotactic response, these activities were one third to one half less than observed with ECGF. These data suggest that ECGF and heparin may play a significant role as response modifiers of human endothelial cell migration which may be relevant to tumor metastasis, wound healing, and atherogenesis.

MeSH Terms
Cells, Cultured Chemotaxis Endothelial Growth Factors Endothelium/physiology Fibroblast Growth Factors/physiology Fibronectins/physiology Growth Substances/immunology,physiology Heparin/physiology Humans Immunologic Techniques Laminin/physiology Umbilical Veins/cytology
Chemicals
Endothelial Growth Factors Fibronectins Growth Substances Laminin Fibroblast Growth Factors Heparin
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Terranova V P
DiFlorio R
Lyall R M
Hic S
Friesel R
Maciag T
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.101.6.2330
Published
1985-12-00
Pages
2330-4
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2114007
Subset
IM
Grants
NIA NIH HHS · AG84807 · United States
NHLBI NIH HHS · HL310765 · United States
External Links
PubMed source DOI: 10.1083/jcb.101.6.2330 PMC Full Text View journal details
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