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PMID: 3913395 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Cloning of DNA sequences from Methanococcus vannielii capable of autonomous replication in yeast.

Archives of microbiology ·Vol. 143 ·No. 3 ·1985-12-00 ·Pages 253-5

Meile L, Leisinger T, Reeve JN

Abstract

Total DNA of the archaebacterium Methanococcus vannielii was digested with BamHI or BamHI/HindIII, cloned with plasmid Yip5 and analyzed for sequences capable of autonomous replication (ARSs) in the eukaryote Saccharomyces cerevisiae. Two recombinant plasmids were isolated which contained 3.3 kb and 8 kb fragments of methanogen derived DNA with ARS activity. They exhibited low transformation efficiencies for yeast and promoted slow growth of yeast transformants.

MeSH Terms
Cloning, Molecular DNA Replication DNA, Bacterial/genetics Euryarchaeota/genetics Plasmids Replicon Saccharomyces cerevisiae/genetics
Chemicals
DNA, Bacterial
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Meile L
Leisinger T
Reeve J N
References (15)
15 references, click to expand
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Article Info
Journal
Archives of microbiology
Abbr.
Arch Microbiol
ISSN
0302-8933
Published
1985-12-00
Pages
253-5
Language
English
Region
Germany
NLM ID
0410427
Subset
IM
Grants
NIA NIH HHS · JK04AG00108 · United States
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