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PMID: 3917980 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

In vitro evidence that human airway lysozyme is cleaved and inactivated by Pseudomonas aeruginosa elastase and not by human leukocyte elastase.

Infection and immunity ·Vol. 47 ·No. 2 ·1985-02-00 ·Pages 555-60

Jacquot J, Tournier JM, Puchelle E

Abstract

The in vitro effects of Pseudomonas aeruginosa elastase (P. aeruginosa E) and of human leukocyte elastase on human airway lysozyme (HAL) were investigated. P. aeruginosa E inactivated and cleaved HAL, whereas human leukocyte elastase had no effect. Total inactivation of HAL by P. aeruginosa E was observed after 120 min of incubation at 37 degrees C, for an elastase-to-lysozyme molar ratio of 1:5. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of reaction mixtures containing HAL and P. aeruginosa E in an elastase-to-lysozyme molar ratio of 1:10 showed a progressive disappearance of the HAL band upon increasing the incubation time with P. aeruginosa E. Gel filtration chromatography indicated that HAL was cleaved into at least three peptide fragments. The cleavage of HAL by P. aeruginosa E was accompanied by parallel losses of its bacteriolytic activity and its immunoreactive property.

MeSH Terms
Bacterial Proteins/metabolism Humans Leukocytes/enzymology Muramidase/metabolism Pancreatic Elastase/metabolism Protein Denaturation Pseudomonas aeruginosa/enzymology,pathogenicity Sputum/enzymology Virulence
Chemicals
Bacterial Proteins Muramidase Pancreatic Elastase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Jacquot J
Tournier J M
Puchelle E
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34 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.47.2.555-560.1985
Published
1985-02-00
Pages
555-60
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC263208
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.47.2.555-560.1985 PMC Full Text View journal details
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