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PMID: 391806 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Spontaneous mutational specificity of drug resistance plasmid pKM101 in Escherichia coli.

Journal of bacteriology ·Vol. 140 ·No. 3 ·1979-12-00 ·Pages 929-37

Fowler RG, McGinty L, Mortelmans KE

Abstract

Plasmid pKM101 enhances the frequency of spontaneous and ultraviolet light-induced mutations in Escherichia coli and protects the cells against the lethal effects of ultraviolet irradiation. By analyzing reversion patterns of defined trpA alleles, we showed that pKM101 caused all types of spontaneous base-pair substitution mutations with the possible exception of guanine . cytosine leads to adenine. thymine transitions. Neither insertion nor deletion frameshift mutations were enhanced. Transversions were more strongly enhanced than transitions, and adenine . thymine base pairs appeared more susceptible to pKM101 mutator activity than guanine . cytosine base pairs. In addition, there were effects from neighboring base pairs and genetic background that influenced the mutator activity of pKM101.

MeSH Terms
Alleles Base Sequence Escherichia coli/drug effects,genetics,metabolism Mutation Nalidixic Acid/pharmacology R Factors Tryptophan/metabolism
Chemicals
Nalidixic Acid Tryptophan
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Fowler R G
McGinty L
Mortelmans K E
References (44)
44 references, click to expand
  1. Detection of carcinogens as mutagens: bacterial tester strains with R factor plasmids.
    Proc Natl Acad Sci U S A. 1975 Mar;72(3):979-83 PMID: 165497
  2. Restoration of mutability in non-mutable Escherichia coli carrying different plasmids.
    Mol Gen Genet. 1977 Oct 24;155(3):287-90 PMID: 202859
  3. Plasmid (pKM101)-mediated enhancement of repair and mutagenesis: dependence on chromosomal genes in Escherichia coli K-12.
    Mol Gen Genet. 1977 Mar 28;152(1):93-103 PMID: 325391
  4. Spontaneous, ultraviolet and ionizing radiation mutagenesis in two auxotrophic strains of Salmonella typhimurium carrying an R plasmid.
    Mutat Res. 1977 Oct;45(1):1-6 PMID: 335234
  5. Isolation and characterization of mutants of the plasmid pKM101 deficient in their ability to enhance mutagenesis and repair.
    J Bacteriol. 1978 Mar;133(3):1203-11 PMID: 346562
  6. R plasmid-mediated enhancement of mutagenesis in strains of Escherichia coli deficient is known repair functions.
    Mutat Res. 1978 Apr;50(1):1-8 PMID: 347278
  7. Molecular basis of base substitution hotspots in Escherichia coli.
    Nature. 1978 Aug 24;274(5673):775-80 PMID: 355893
  8. Segregation of the mutator property of plasmid R46 from its ultraviolet-protecting property.
    Mol Gen Genet. 1979 Jan 2;167(3):317-27 PMID: 368596
  9. Effect of host lex, recA, recF, and uvrD genotypes on the ultraviolet light-protecting and related properties of plasmid R46 in Escherichia coli.
    J Bacteriol. 1979 Feb;137(2):830-8 PMID: 370103
  10. The relation of repair phenomena to mutation induction in bacteria.
    Mutat Res. 1978;55(2):85-120 PMID: 372787
  11. The effects of the ultraviolet-protecting plasmids pKM101 and R205 on DNA polymerase I activity in Escherichia coli K-12.
    Mutat Res. 1979 Apr;60(2):135-42 PMID: 379622
  12. Expression of the plasmid pKM101--determined DNA repair system in recA- and lex- strains of Escherichia coli.
    Mol Gen Genet. 1976 Jun 15;145(3):303-6 PMID: 781517
  13. Ultraviolet light protection, enhancement of ultraviolet light mutagenesis, and mutator effect of plasmid R46 in Salmonella typhimurium.
    J Bacteriol. 1976 Oct;128(1):271-82 PMID: 789333
  14. Ultraviolet mutagenesis and inducible DNA repair in Escherichia coli.
    Bacteriol Rev. 1976 Dec;40(4):869-907 PMID: 795416
  15. Bacterial mutator genes and the control of spontaneous mutation.
    Annu Rev Genet. 1976;10:135-56 PMID: 797306
  16. Genetic and kinetic evidence for different types of postreplication repair in Escherichia coli B.
    J Bacteriol. 1975 Jul;123(1):154-61 PMID: 1095549
  17. SOS repair hypothesis: phenomenology of an inducible DNA repair which is accompanied by mutagenesis.
    Basic Life Sci. 1975;5A:355-67 PMID: 1103845
  18. Uniform nomenclature for bacterial plasmids: a proposal.
    Bacteriol Rev. 1976 Mar;40(1):168-89 PMID: 1267736
  19. Effect of an R factor on resistance of Salmonella typhimurium to radiation and chemical treatment.
    Mutat Res. 1972 Apr;14(4):450-3 PMID: 4552776
  20. Mutator gene studies in Escherichia coli: the mutS gene.
    Genetics. 1972 Dec;72(4):551-67 PMID: 4569178
  21. Effects of an R factor and caffeine on ultraviolet mutability in Salmonella typhimurium.
    Mutat Res. 1973 Jun;18(3):367-70 PMID: 4576319
  22. Independent expression of the A gene of the tryptophan operon of Escherichia coli during tryptophan starvation.
    J Gen Microbiol. 1973 Jun;76(2):395-405 PMID: 4579131
  23. Conditional mutator gene in Escherichia coli: isolation, mapping, and effector studies.
    J Bacteriol. 1974 Feb;117(2):477-87 PMID: 4590472
  24. Thermal enhancement of ultraviolet mutability in a tif-1 uvrA derivative of Escherichia coli B-r: evidence that ultraviolet mutagenesis depends upon an inducible function.
    Proc Natl Acad Sci U S A. 1974 May;71(5):1930-4 PMID: 4600265
  25. DNA polymerase activity determined by the ultraviolet-protecting plasmid, R-Utrecht.
    Nature. 1974 Oct 4;251(5474):432-4 PMID: 4608923
  26. Letter to the editor: Characterization of mutations in the tryptophan operon of Escherichia coli by RNA nucleotide sequencing.
    J Mol Biol. 1974 Oct 5;88(4):913-5 PMID: 4610162
  27. Mutational specificity of a conditional Escherichia coli mutator, mutD5.
    Mol Gen Genet. 1974;133(3):179-91 PMID: 4614067
  28. Amino acid replacements and the genetic code.
    Cold Spring Harb Symp Quant Biol. 1966;31:151-62 PMID: 4866372
  29. Altered amino acid sequences produced by reversion of frameshift mutants of tryptophan synthetase A gene of E. coli.
    Proc Natl Acad Sci U S A. 1967 Oct;58(4):1499-506 PMID: 4867662
  30. Analysis of amino acid replacements resulting from frameshift and missense mutations in the tryptophan synthetase A gene of Escherichia coli.
    J Mol Biol. 1968 Jul 14;34(2):219-38 PMID: 4938546
  31. Amino acid replacements of the glutamic acid residue at position 48 in the tryptophan synthetase A protein of Escherichia coli.
    J Mol Biol. 1968 Jul 28;35(2):357-67 PMID: 4939784
  32. Normal and mutant glycine transfer RNAs.
    Nat New Biol. 1971 Oct 27;233(43):274-7 PMID: 4941781
  33. Analysis of DNA polymerases II and 3 in mutants of Escherichia coli thermosensitive for DNA synthesis.
    Proc Natl Acad Sci U S A. 1971 Dec;68(12):3150-3 PMID: 4943556
  34. Resistance to the bactericidal effect of ultraviolet radiation conferred on Enterobacteria by the colicine factor coli.
    J Gen Microbiol. 1965 Jul;40(1):43-55 PMID: 5323082
  35. Increase in frequency of ultraviolet-induced mutation brought about by the colicine factor, col-I in Salmonella typhimurium.
    Mutat Res. 1966 Apr;3(2):129-34 PMID: 5333391
  36. A proposal for a uniform nomenclature in bacterial genetics.
    Genetics. 1966 Jul;54(1):61-76 PMID: 5961488
  37. Acetylornithinase of Escherichia coli: partial purification and some properties.
    J Biol Chem. 1956 Jan;218(1):97-106 PMID: 13278318
  38. Influence of 3-methylanthranilic and anthranilic acids on the formation of tryptophan synthetase in Escherichia coli.
    J Bacteriol. 1961 Jan;81:81-90 PMID: 13761197
  39. A genetic and biochemical analysis of second site reversion.
    J Biol Chem. 1963 Mar;238:1043-8 PMID: 13953567
  40. A BIOCHEMICAL AND GENETIC STUDY OF REVERSION WITH THE A-GENE A-PROTEIN SYSTEM OF ESCHERICHIA COLI TRYPTOPHAN SYNTHETASE.
    Genetics. 1963 Aug;48:1065-83 PMID: 14050802
  41. ON THE COLINEARITY OF GENE STRUCTURE AND PROTEIN STRUCTURE.
    Proc Natl Acad Sci U S A. 1964 Feb;51:266-72 PMID: 14124325
  42. PROTEIN STRUCTURE RELATIONSHIPS REVEALED BY MUTATIONAL ANALYSIS.
    Science. 1964 Dec 18;146(3651):1593-4 PMID: 14224506
  43. Mapping of rfa Genes in Salmonella typhimurium by ES18 and P22 Transduction and by Conjugation.
    J Bacteriol. 1972 Oct;112(1):48-57 PMID: 16559160
  44. THE COMPLETE AMINO ACID SEQUENCE OF THE TRYPTOPHAN SYNTHETASE A PROTEIN (alpha SUBUNIT) AND ITS COLINEAR RELATIONSHIP WITH THE GENETIC MAP OF THE A GENE.
    Proc Natl Acad Sci U S A. 1967 Feb;57(2):296-8 PMID: 16591468
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.140.3.929-937.1979
Published
1979-12-00
Pages
929-37
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC216735
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.140.3.929-937.1979 PMC Full Text View journal details
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