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PMID: 3919706 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Monoclonal antibodies to a phosphoprotein from chromatin of rat liver.

The Biochemical journal ·Vol. 225 ·No. 2 ·1985-01-15 ·Pages 357-63

Halikowski MJ, Liew CC

Abstract

Three monoclonal antibody subclasses (IgG1, IgG2a, and IgM) were raised to the phosphoprotein B2 (Mr 68000, pI6.5-8.2) which has been shown previously to be associated with the nucleosomes of rat liver nuclei. These antibodies do not show any significant cross reactivity with CM-cellulose 'unbound' non-histone chromosomal proteins, bovine serum albumin or histones. Further verification of the specificity of these antibodies to this phosphoprotein was carried out using both 'dot' blot and immunological transfer analysis ('Western blot'). The monoclonal antibodies (IgG1 and IgG2a) could also be used to semi-quantify the phosphoprotein B2 in rat liver nuclei. The high specificity and unlimited availability of this type of probe provides a means to study the role(s) of this phosphoprotein in the overall scheme of actively transcribed chromatin.

MeSH Terms

Animals Antibodies, Monoclonal/immunology Chromatin/immunology Chromosomal Proteins, Non-Histone/immunology,isolation & purification Immunodiffusion Immunoelectrophoresis Immunoglobulin G/immunology Liver/immunology Male Phosphoproteins/immunology,isolation & purification Radioimmunoassay Rats Rats, Inbred Strains

Chemicals

Antibodies, Monoclonal Chromatin Chromosomal Proteins, Non-Histone Immunoglobulin G Phosphoproteins nuclear phosphoprotein B2, rat

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Halikowski M J

Liew C C

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27 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj2250357

Published

1985-01-15

Pages

357-63

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1144598

Subset

Grants

NHLBI NIH HHS · HL-18824-01 · United States

External Links

PubMed source DOI: 10.1042/bj2250357 PMC Full Text View journal details

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Monoclonal antibodies to a phosphoprotein from chromatin of rat liver.

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