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PMID: 3920146 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Adhesion of a human fecal Escherichia coli strain to mouse colonic mucus.

Infection and immunity ·Vol. 48 ·No. 1 ·1985-04-00 ·Pages 139-45

Cohen PS, Arruda JC, Williams TJ, Laux DC

Abstract

Escherichia coli F-18 isolated from the feces of a healthy human is an excellent colonizer of the CD-1 mouse colon. In the present investigation, adhesion of E. coli F-18 to CD-1 mouse colonic mucus and bovine serum albumin (BSA), immobilized on polystyrene, was studied. Adhesion of E. coli F-18 to mucus was two- to sixfold greater than to either BSA or polystyrene. E. coli F-18 lipopolysaccharide specifically blocked adhesion of E. coli F-18 to mucus and mimicked adhesion of E. coli F-18 to mucus, BSA, and polystyrene. Purified capsule also blocked adhesion of E. coli F-18 to mucus, but this inhibition was found to be entirely nonspecific. The specific E. coli F-18 receptor in mucus appeared to be a glycoprotein, containing sugars normally found in mucins and having a maximum molecular weight of between 1.25 X 10(5) and 2.5 X 10(5).

MeSH Terms
Adhesiveness Animals Colon/microbiology Edetic Acid/pharmacology Escherichia coli/physiology Feces/microbiology Glycoproteins/analysis Humans Lipopolysaccharides/pharmacology Male Mice Mice, Inbred Strains Molecular Weight Mucus/analysis,microbiology
Chemicals
Glycoproteins Lipopolysaccharides Edetic Acid
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Cohen P S
Arruda J C
Williams T J
Laux D C
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24 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.48.1.139-145.1985
Published
1985-04-00
Pages
139-45
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC261926
Subset
IM
Grants
NIAID NIH HHS · AI16370 · United States
External Links
PubMed source DOI: 10.1128/iai.48.1.139-145.1985 PMC Full Text View journal details
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