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PMID: 392516 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Cloning and expression of the Escherichia coli replication origin in a single-stranded DNA phage.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 12 ·1979-12-00 ·Pages 6250-4

Kaguni J, LaVerne LS, Ray DS

Abstract

The Escherichia coli DNA replication origin (oriC) and the adjacent asparagine synthetase gene (asnA) have been inserted into the duplex replicative form DNA of the single-stranded phage vector M13Goril. By in vitro recombination, the entire oriC asnA-containing plasmid pJS5 was inserted into M13Gori1 in both possible orientations. Both phage types transduce the asnA gene and confer upon the M13 vector the ability to replicate as a plasmid in the E. coli mutant rep3. In rep+ hosts, these phages undergo single-stranded DNA synthesis and viral morphogenesis.

MeSH Terms
Cloning, Molecular Coliphages/genetics DNA Replication DNA Restriction Enzymes DNA, Bacterial/genetics DNA, Recombinant DNA, Single-Stranded/biosynthesis DNA, Viral/biosynthesis Escherichia coli/genetics Mutation Plasmids
Chemicals
DNA, Bacterial DNA, Recombinant DNA, Single-Stranded DNA, Viral DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kaguni J
LaVerne L S
Ray D S
References (29)
29 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.76.12.6250
Published
1979-12-00
Pages
6250-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC411841
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.76.12.6250 PMC Full Text View journal details
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