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PMID: 3926821 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Differentiation and activation phenotypes of lung T lymphocytes differ from those of circulating T lymphocytes.

The Journal of clinical investigation ·Vol. 76 ·No. 1 ·1985-07-00 ·Pages 60-5

Davidson BL, Faust J, Pessano S, Daniele RP, Rovera G

Abstract

We used dual laser two-color flow cytometry to compare the expression of surface markers associated with activation and with differentiation in lung and peripheral blood T lymphocytes from normal subjects. T cell subsets, defined based on their reactivity with monoclonal antibodies (MAb) OKT3, OKT4, and OKT8, were analyzed for expression of activation antigens as detected by MAbs to the interleukin-2 receptor, the transferrin receptor, and HLA-DR determinants. Whereas circulating T lymphocytes expressed the three activation antigens at low levels, and the total of T4+ and T8+ cells always approximated the number of T3+ cells, lung T lymphocytes of the T3+, T4+, and T8+ populations expressed the activation antigens at variable levels in combinations not seen in circulating lymphocytes, and the sum of T4+ and T8+ cells always exceeded the T3+ total. A proportion of T4+T8+ cells was detected in lung lymphocytes.

MeSH Terms
Adult Antibodies, Monoclonal Antigens, Differentiation, T-Lymphocyte Antigens, Surface/analysis Cell Cycle Female Flow Cytometry HLA-DR Antigens Histocompatibility Antigens Class II/analysis Humans Lung/cytology Lymphocyte Activation Male Receptors, Immunologic/analysis Receptors, Interleukin-2 T-Lymphocytes/immunology
Chemicals
Antibodies, Monoclonal Antigens, Differentiation, T-Lymphocyte Antigens, Surface HLA-DR Antigens Histocompatibility Antigens Class II Receptors, Immunologic Receptors, Interleukin-2
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Davidson B L
Faust J
Pessano S
Daniele R P
Rovera G
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37 references, click to expand
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI111977
Published
1985-07-00
Pages
60-5
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC423706
Subset
IM
Grants
NHLBI NIH HHS · HL-07000 · United States
External Links
PubMed source DOI: 10.1172/JCI111977 PMC Full Text View journal details
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