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PMID: 3928493 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Recombinant and natural gamma-interferon activation of macrophages in vitro: different dose requirements for induction of killing activity against phagocytizable and nonphagocytizable fungi.

Infection and immunity ·Vol. 49 ·No. 3 ·1985-09-00 ·Pages 724-30

Brummer E, Morrison CJ, Stevens DA

Abstract

Recombinant murine gamma-interferon (IFN) and supernatants from concanavalin A (ConA)-stimulated spleen cells were tested for their ability to activate resident peritoneal macrophages (M phi) for fungicidal activity. M phi monolayers pulsed overnight with IFN exhibited significantly enhanced fungicidal activity against Candida albicans (44 +/- 12 versus 0.0%) and Blastomyces dermatitidis (34 +/- 1 versus 3 +/- 3%). The effect of IFN was dose dependent; however, less IFN (10 U/ml) was required to activate M phi to kill phagocytizable C. albicans than to kill nonphagocytizable B. dermatitidis (1,000 U/ml). ConA-stimulated spleen cell supernatants were also able to activate M phi for fungicidal activity against both fungi. The capacity of ConA-stimulated spleen cell supernatants to activate M phi for fungicidal activity was neutralized in the presence of antibody to murine IFN. ConA-treated monolayers acquired the ability to kill C. albicans, but not B. dermatitidis, which was shown to be associated with residual (10%) lymphocytes in the monolayers. Lipopolysaccharide (0.001 to 10 micrograms/ml) failed to consistently activate M phi for fungicidal activity. These data show that IFN can exert an immunoregulatory role on M phi defense against these fungal pathogens.

MeSH Terms
Animals Antibodies/immunology Concanavalin A/pharmacology DNA, Recombinant Dose-Response Relationship, Drug Fungi/immunology In Vitro Techniques Interferon-gamma/pharmacology Lipopolysaccharides/pharmacology Macrophage Activation/drug effects Male Mice Mice, Inbred BALB C Phagocytosis/drug effects
Chemicals
Antibodies DNA, Recombinant Lipopolysaccharides Concanavalin A Interferon-gamma
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Brummer E
Morrison C J
Stevens D A
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.49.3.724-730.1985
Published
1985-09-00
Pages
724-30
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC261257
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.49.3.724-730.1985 PMC Full Text View journal details
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