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PMID: 3933484 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The sporulation-specific penicillin-binding protein 5a from Bacillus subtilis is a DD-carboxypeptidase in vitro.

The Biochemical journal ·Vol. 230 ·No. 3 ·1985-09-15 ·Pages 825-8

Todd JA, Bone EJ, Ellar DJ

Abstract

The sporulation-specific penicillin-binding protein 5a was purified from Bacillus subtilis and shown to possess dd-carboxypeptidase activity in vitro.

MeSH Terms
Bacillus subtilis/enzymology,physiology Bacterial Proteins Carboxypeptidases/metabolism Carrier Proteins/isolation & purification,metabolism Cell Membrane/enzymology Chromatography, Affinity Electrophoresis, Polyacrylamide Gel Hexosyltransferases Muramoylpentapeptide Carboxypeptidase/isolation & purification,metabolism Penicillin-Binding Proteins Peptidyl Transferases Spores, Bacterial/enzymology
Chemicals
Bacterial Proteins Carrier Proteins Penicillin-Binding Proteins Peptidyl Transferases Hexosyltransferases Carboxypeptidases Muramoylpentapeptide Carboxypeptidase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Todd J A
Bone E J
Ellar D J
References (12)
12 references, click to expand
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  3. Isolation by covalent affinity chromatography of the penicillin-binding components from membranes of Bacillus subtilis.
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  4. Heat resistance of bacterial endospores and concept of an expanded osmoregulatory cortex.
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  6. The interaction of nocardicin A with the penicillin-binding proteins of Bacillus megaterium KM.
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  7. Commitment of bacterial spores to germinate. A measure of the trigger reaction.
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  8. Alteration in the penicillin-binding profile of Bacillus megaterium during sporulation.
    Nature. 1982 Dec 16;300(5893):640-3 PMID: 6815541
  9. Changes in penicillin-binding proteins during sporulation of Bacillus subtilis.
    J Bacteriol. 1983 Mar;153(3):1331-7 PMID: 6402492
  10. Construction and properties of an integrable plasmid for Bacillus subtilis.
    J Bacteriol. 1983 Jun;154(3):1513-5 PMID: 6304019
  11. Identification of penicillin-binding protein 5a of Bacillus megaterium KM as a DD-carboxypeptidase.
    Biochem J. 1983 Aug 15;214(2):653-5 PMID: 6412705
  12. Cleavage of a COOH-terminal hydrophobic region from D-alanine carboxypeptidase, a penicillin-sensitive bacterial membrane enzyme. Characterization of active, water-soluble fragments.
    J Biol Chem. 1979 Jun 10;254(11):4863-75 PMID: 438218
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2300825
Published
1985-09-15
Pages
825-8
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1152690
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2300825 PMC Full Text View journal details
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