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PMID: 3936020 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Differential inhibition of human placental DNA polymerases delta and alpha by BuPdGTP and BuAdATP.

Nucleic acids research ·Vol. 13 ·No. 23 ·1985-12-09 ·Pages 8623-30

Lee MY, Toomey NL, Wright GE

Abstract

The p-n-butylphenyl- and p-n-butylanilino- substituted analogs of dGTP and dATP, respectively, were tested as inhibitors of purified human placental DNA polymerases alpha and delta. It was observed that DNA polymerase alpha activity was potently inhibited by these analogs with I0.5 values as low as the nanomolar range, whereas DNA polymerase delta activity was poorly inhibited, with I0.5 values of ca. 100 micromolar. These results argue for a distinct identity of these two enzymes, and demonstrate the usefulness of these analogs as probes of DNA polymerase structures. In addition, these analogs provide a rapid method for the discrimination of the two enzyme activities and a means for the selective assay of DNA polymerase delta. Aphidicolin inhibited both DNA polymerases.

MeSH Terms
Adenosine Triphosphate/analogs & derivatives,pharmacology Aphidicolin Bacillus subtilis/enzymology DNA/biosynthesis DNA Polymerase II/antagonists & inhibitors DNA Polymerase III/antagonists & inhibitors Deoxyguanine Nucleotides/pharmacology Diterpenes/pharmacology Dose-Response Relationship, Drug Female Humans Nucleic Acid Synthesis Inhibitors Placenta/enzymology Pregnancy Templates, Genetic
Chemicals
Deoxyguanine Nucleotides Diterpenes Nucleic Acid Synthesis Inhibitors Aphidicolin N(2)-(4-n-butylphenyl)-2'-deoxyguanosine 5'-triphosphate Adenosine Triphosphate DNA 4-n-butylanilino dATP DNA Polymerase II DNA Polymerase III
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lee M Y
Toomey N L
Wright G E
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23 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/13.23.8623
Published
1985-12-09
Pages
8623-30
Language
English
Region
England
NLM ID
0411011
PMCID
PMC322157
Subset
IM
Grants
NIGMS NIH HHS · GM 31973 · United States
External Links
PubMed source DOI: 10.1093/nar/13.23.8623 PMC Full Text View journal details
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