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PMID: 3939320 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Selective translational regulation of ribosomal protein gene expression during early development of Drosophila melanogaster.

Molecular and cellular biology ·Vol. 5 ·No. 12 ·1985-12-00 ·Pages 3583-92

Kay MA, Jacobs-Lorena M

Abstract

We have previously characterized a cloned cDNA coding for a developmentally regulated mRNA in Drosophila melanogaster whose expression is selectively regulated at the translational level during oogenesis and embryogenesis. In this report we show that this translationally regulated mRNA (rpA1) codes for an acidic ribosomal protein. Furthermore, our results indicate that most ribosomal protein mRNAs are regulated similarly to rpA1 mRNA. This conclusion is based on cell-free translation of mRNAs derived from polysomes and postpolysomal supernatants as well as in vivo labeling experiments. Thus, the translation of many ribosomal protein mRNAs appears to be temporally related to the synthesis of rRNA during D. melanogaster development. The relationship between rRNA transcription and ribosomal protein mRNA translation was further investigated by genetically reducing rRNA synthesis with the use of bobbed mutants. Unexpectedly, neither ribosomal protein mRNA abundance nor translation was altered in these mutants.

MeSH Terms
Animals Drosophila melanogaster/genetics,growth & development,metabolism Female Gene Expression Regulation Mutation Ovary/metabolism Polyribosomes/metabolism Protein Biosynthesis RNA, Messenger/genetics RNA, Ribosomal/genetics Ribosomal Proteins/biosynthesis,genetics
Chemicals
RNA, Messenger RNA, Ribosomal Ribosomal Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kay M A
Jacobs-Lorena M
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47 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.5.12.3583-3592.1985
Published
1985-12-00
Pages
3583-92
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC369189
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.5.12.3583-3592.1985 PMC Full Text View journal details
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