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PMID: 395018 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Escherichia coli K-12 auxotrophs induced by insertion of the transposable element Tn5.

Genetics ·Vol. 92 ·No. 3 ·1979-07-00 ·Pages 741-7

Shaw KJ, Berg CM

Abstract

The sites of insertion of the transposable kanamycin-neomycin resistance-determining element, Tn5, in the E. coli K-12 chromosome were assessed in a collection of over 300 auxotrophs. Although mutations in at least 45 different cistrons were obtained, the distribution of insertion sites was not completely random: proA or proB; cysG; and cysH, cysD or cysC mutants were found in excess.

MeSH Terms
Chromosome Mapping Chromosomes, Bacterial DNA Transposable Elements Drug Resistance, Microbial Escherichia coli/genetics Kanamycin/pharmacology Mutation Neomycin/pharmacology Recombination, Genetic
Chemicals
DNA Transposable Elements Kanamycin Neomycin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Shaw K J
Berg C M
References (12)
12 references, click to expand
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/92.3.741
Published
1979-07-00
Pages
741-7
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1214033
Subset
IM
External Links
PubMed source DOI: 10.1093/genetics/92.3.741 PMC Full Text View journal details
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