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PMID: 3960717 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Sequence heterogeneity within the human alphoid repetitive DNA family.

Nucleic acids research ·Vol. 14 ·No. 5 ·1986-03-11 ·Pages 2059-73

Devilee P, Slagboom P, Cornelisse CJ, Pearson PL

Abstract

We have cloned and determined the base-sequence and genome organization of two human chromosome-specific alphoid DNA fragments, designated L1.26, mapping principally to chromosomes 13 and 21, and L1.84, mapping to chromosome 18. Their copy number is estimated to be approximately 2,000 per haploid genome. L1.84 has a double-dimer organization, whereas L1.26 has a much less defined higher order tandem organization. Further, we present evidence that the restriction-site spacing within the alphoid DNA family is chromosome specific. From sequence analysis, clones L1.26 and L1.84 are found to consist of 5 and 4 tandemly duplicated 170 bp monomers. Cross-homology between the various monomers is 65-85%. The analysis suggests that the evolution of tandem-arrays does not take place via a defined 340 bp unit, as was inferred by others, but via circularly permutated monomers or multimers of the 170 bp unit.

MeSH Terms
Base Sequence Biological Evolution Centromere/ultrastructure Chromosome Mapping Chromosomes/ultrastructure Chromosomes, Human, 13-15 Chromosomes, Human, 21-22 and Y Cloning, Molecular DNA/genetics Humans Repetitive Sequences, Nucleic Acid Sequence Homology, Nucleic Acid X Chromosome
Chemicals
DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Devilee P
Slagboom P
Cornelisse C J
Pearson P L
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29 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/14.5.2059
Published
1986-03-11
Pages
2059-73
Language
English
Region
England
NLM ID
0411011
PMCID
PMC339643
Subset
IM
Databases
GENBANK
X03692, X03693
External Links
PubMed source DOI: 10.1093/nar/14.5.2059 PMC Full Text View journal details
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