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PMID: 3960732 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Salt induced B----A transition of poly(dG).poly(dC) and the stabilization of A form by its methylation.

Nucleic acids research ·Vol. 14 ·No. 6 ·1986-03-25 ·Pages 2737-48

Nishimura Y, Torigoe C, Tsuboi M

Abstract

Raman spectra of poly(dG).poly(dC) have been observed in aqueous solutions at various ionic strengths, [NaCl] = 0.03 to 4 M, and at different temperatures, 10 to 60 degrees C. At 30 degrees C, and at [NaCl] = 0.03 M, it was found to have a B-form (with O4'endo-anti guanosine and C2'endo-anti cytidine), whereas, at [NaCl] = 4 M, an A form (with C3'endo-anti guanosine and C3'endo-anti cytidine). At 30 degrees C and [NaCl] = 1 M, namely at an intermediate state, a fraction of this molecules was considered to have a "heteronomous A" form (with O4'endo-anti guanosine and C3' endo-anti cytidine). At 60 degrees C and [NaCl] = 1 M, it assumes the B form, and at 10 degrees C and [NaCl] = 1 M, the A form. Cytosine-5-methylation was found to cause a marked stabilization of the A form. Even at [NaCl] = 0.1 M (at 30 degrees C), a substantial portion of poly(dG).poly(dm5C) was found to have a heteronomous form, in which the dG atrand is in the B form and the dC an A form; it never assumes a complete B form.

MeSH Terms
Methylation Nucleic Acid Conformation Polydeoxyribonucleotides Salts Spectrum Analysis, Raman
Chemicals
Polydeoxyribonucleotides Salts poly(dC-dG)
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Nishimura Y
Torigoe C
Tsuboi M
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25 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/14.6.2737
Published
1986-03-25
Pages
2737-48
Language
English
Region
England
NLM ID
0411011
PMCID
PMC339695
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/14.6.2737 PMC Full Text View journal details
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