Purification of rabbit tumor necrosis factor.

FEBS letters ·Vol. 180 ·No. 2 ·1985-01-28 ·Pages 203-6

Abe S, Gatanaga T, Yamazaki M, Soma G, Mizuno D

Abstract

Rabbit tumor necrosis factor (TNF) was purified and shown by SDS-PAGE to be a single protein of 18 kDa. TNF in 355 ml rabbit serum was precipitated with ammonium sulfate, and purified by repeated DEAE-Sephadex and Sephacryl S-200 chromatographies, and the final fractionation on Blue-Sepharose 6B. By this procedure its yield was 22% and its specific activity was 2.4 X 10(7) U/mg protein. The sequence of the N-terminal 20 amino acids was determined.

MeSH Terms

Amino Acid Sequence Animals Chromatography, Gel Chromatography, High Pressure Liquid Chromatography, Ion Exchange Electrophoresis, Polyacrylamide Gel Glycoproteins/isolation & purification Molecular Weight Rabbits Tumor Necrosis Factor-alpha

Chemicals

Glycoproteins Tumor Necrosis Factor-alpha

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Abe S

Gatanaga T

Yamazaki M

Soma G

Mizuno D

Article Info

Journal

FEBS letters

Abbr.

FEBS Lett

ISSN

0014-5793

DOI

10.1016/0014-5793(85)81071-1

Published

1985-01-28

Pages

203-6

Language

English

Region

England

NLM ID

0155157

Subset

External Links

PubMed source DOI: 10.1016/0014-5793(85)81071-1 View journal details

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Purification of rabbit tumor necrosis factor.

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