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PMID: 3967921 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Iron-vibriobactin transport system is not required for virulence of Vibrio cholerae.

Infection and immunity ·Vol. 47 ·No. 2 ·1985-02-00 ·Pages 360-2

Sigel SP, Stoebner JA, Payne SM

Abstract

The possible requirement of a functional siderophore (vibriobactin)-mediated iron transport system in the pathogenicity of Vibrio cholerae was determined. Two mutants of V. cholerae defective in the iron-vibriobactin transport system were examined for their ability to multiply and elicit diarrhea in infant mice. One mutant, 40130, was unable to synthesize vibriobactin. The second mutant, 1510, was able to synthesize, but not transport, the siderophore. Both mutants retained the ability to multiply and produce disease in the infant mouse, and virulence was indistinguishable from the parent strains. This indicates that a functional iron-vibriobactin transport system is not essential for cholera pathogenesis. These mutants, like the wild-type strains, were found to have a ferric citrate iron uptake system and could utilize citrate or asparagine for growth in low-iron medium. Compounds of this type may increase the availability of iron to V. cholerae in the host.

MeSH Terms
Animals Asparagine/metabolism Biological Transport, Active Catechols/metabolism,physiology Citrates/metabolism Iron/metabolism Mice Oxazoles Vibrio cholerae/genetics,metabolism,pathogenicity Virulence
Chemicals
Catechols Citrates Oxazoles Asparagine vibriobactin Iron
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Sigel S P
Stoebner J A
Payne S M
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21 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.47.2.360-362.1985
Published
1985-02-00
Pages
360-2
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC263175
Subset
IM
Grants
NIAID NIH HHS · AI16935 · United States
External Links
PubMed source DOI: 10.1128/iai.47.2.360-362.1985 PMC Full Text View journal details
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