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PMID: 3980018 Published · ppublish English Journal Article

DNA probe localization at 18p113 band by in situ hybridization and identification of a small supernumerary chromosome.

Human genetics ·Vol. 69 ·No. 3 ·1985-00-00 ·Pages 268-71

Mattei MG, Philip N, Passage E, Moisan JP, Mandel JL, Mattei JF

Abstract

Recombinant plasmid clone B74 (also named D18 S3) containing a human single-copy DNA segment of 6 kilobases (kb) was localized by in situ hybridization on band p113 of chromosome 18. This probe was then used in cytogenetic diagnosis to identify precisely a small supernumerary chromosome as an isochromosome i(18p).

MeSH Terms
Autoradiography Cells, Cultured Chromosome Aberrations Chromosome Banding Chromosomes, Human, 16-18 Cloning, Molecular DNA/genetics Female Humans Intellectual Disability/genetics Karyotyping Lymphocytes/ultrastructure Male Nucleic Acid Hybridization Trisomy
Chemicals
DNA
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Mattei M G
Philip N
Passage E
Moisan J P
Mandel J L
Mattei J F
References (12)
12 references, click to expand
  1. Localization of the beta-globin gene by chromosomal in situ hybridization.
    Am J Hum Genet. 1984 May;36(3):576-85 PMID: 6587773
  2. Localization of the human alpha-globin gene cluster to the short arm of chromosome 16 (16p12-16pter) by hybridization in situ.
    J Mol Biol. 1982 Apr 5;156(2):269-78 PMID: 6177863
  3. Localization of a unique gene by direct hybridization in situ.
    Proc Natl Acad Sci U S A. 1981 Jun;78(6):3755-9 PMID: 6943581
  4. Chromosomal localization of the human placental lactogen-growth hormone gene cluster to 17q22-24.
    Am J Hum Genet. 1982 Mar;34(2):227-34 PMID: 7072716
  5. Localization of the restriction fragment length polymorphism D14S1 (pAW-101) to chromosome 14q32.1 leads to 32.2 by in situ hybridization.
    Am J Hum Genet. 1983 Nov;35(6):1097-106 PMID: 6650497
  6. Localization of single copy DNA sequences of G-banded human chromosomes by in situ hybridization.
    Chromosoma. 1981;83(3):431-9 PMID: 7273954
  7. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.
    Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683-7 PMID: 291033
  8. Patterns of DNA replication of human chromosomes. II. Replication map and replication model.
    Am J Hum Genet. 1982 Sep;34(5):757-80 PMID: 7124731
  9. High-resolution chromosomal localization of human genes for amylase, proopiomelanocortin, somatostatin, and a DNA fragment (D3S1) by in situ hybridization.
    Proc Natl Acad Sci U S A. 1983 Nov;80(22):6932-6 PMID: 6196780
  10. Localization of the thyroglobulin gene by in situ hybridization to human chromosomes.
    Hum Genet. 1985;69(1):28-31 PMID: 3967888
  11. New Giemsa method for the differential staining of sister chromatids.
    Nature. 1974 Sep 13;251(5471):156-8 PMID: 4138930
  12. Chromosomal localization of a single copy gene by in situ hybridization--human beta globin genes on the short arm of chromosome 11.
    Ann Hum Genet. 1981 May;45(Pt 2):135-41 PMID: 6172077
Article Info
Journal
Human genetics
Abbr.
Hum Genet
ISSN
0340-6717
DOI
10.1007/BF00293038
Published
1985-00-00
Pages
268-71
Language
English
Region
Germany
NLM ID
7613873
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00293038 View journal details
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