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PMID: 3980099 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Identification and characterization of Vibrio cholerae surface proteins by radioiodination.

Infection and immunity ·Vol. 48 ·No. 1 ·1985-04-00 ·Pages 87-93

Richardson K, Parker CD

Abstract

Whole cells and isolated outer membrane from Vibrio cholerae (Classical, Inaba) were radiolabeled with Iodogen or Iodo-beads as catalyst. Radiolabeling of whole cells was shown to be surface specific by sodium dodecyl sulfate-urea polyacrylamide gel electrophoresis of whole cells and cell fractions. Surface-labeled whole cells regularly showed 16 distinguishable protein species, of which nine were found in radiolabeled outer membrane preparations obtained by a lithium chloride-lithium acetate procedure. Eight of these proteins were found in outer membranes prepared by sucrose density gradient centrifugation and Triton X-100 extraction of radiolabeled whole cells. The mobility of several proteins was shown to be affected by temperature, and the major protein species exposed on the cell surface was shown to consist of at least two different peptides.

MeSH Terms
Bacterial Outer Membrane Proteins/analysis Electrophoresis, Polyacrylamide Gel Hot Temperature Iodine Radioisotopes Lithium/pharmacology Vibrio cholerae/analysis
Chemicals
Bacterial Outer Membrane Proteins Iodine Radioisotopes Lithium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Richardson K
Parker C D
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33 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.48.1.87-93.1985
Published
1985-04-00
Pages
87-93
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC261919
Subset
IM
Grants
NIAID NIH HHS · AI 17669 · United States
External Links
PubMed source DOI: 10.1128/iai.48.1.87-93.1985 PMC Full Text View journal details
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