Calcium transport mechanisms in basolateral plasma membrane-enriched vesicles from rat parotid gland.

The Biochemical journal ·Vol. 227 ·No. 1 ·1985-04-01 ·Pages 239-45

Takuma T, Kuyatt BL, Baum BJ

Abstract

Ca2+ transport was studied by using basolateral plasma membrane vesicles from rat parotid gland prepared by a Percoll gradient centrifugation method. In these membrane vesicles, there were two Ca2+ transport systems; Na+/Ca2+ exchange and ATP-dependent Ca2+ transport. An outwardly directed Na+ gradient increased Ca2+ uptake. Ca2+ efflux from Ca2+-preloaded vesicles was stimulated by an inwardly directed Na+ gradient. However, Na+/Ca2+ exchange did not show any 'uphill' transport of Ca2+ against its own gradient. ATP-dependent Ca2+ transport exhibited 'uphill' transport. An inwardly directed Na+ gradient also decreased Ca2+ accumulation by ATP-dependent Ca2+ uptake. The inhibition of Ca2+ accumulation was proportional to the external Na+ level. Na+/Ca2+ exchange was inhibited by monensin, tetracaine and chlorpromazine, whereas ATP-dependent Ca2+ transport was inhibited by orthovanadate, tetracaine and chlorpromazine. Oligomycin had no effect on either system. These results suggest that in the parotid gland cellular free Ca2+ is extruded mainly by an ATP-dependent Ca2+ transport system, and Na+/Ca2+ exchange may modify the efficacy of that system.

MeSH Terms

Adenosine Triphosphate/pharmacology Animals Antimetabolites/pharmacology Biological Transport/drug effects Calcium/metabolism Cell Membrane/drug effects,metabolism In Vitro Techniques Male Membrane Proteins/metabolism Parotid Gland/metabolism Rats Rats, Inbred Strains Sodium/metabolism

Chemicals

Antimetabolites Membrane Proteins Adenosine Triphosphate Sodium Calcium

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Takuma T

Kuyatt B L

Baum B J

References (24)

24 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj2270239

Published

1985-04-01

Pages

239-45

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1144832

Subset

External Links

PubMed source DOI: 10.1042/bj2270239 PMC Full Text View journal details

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Calcium transport mechanisms in basolateral plasma membrane-enriched vesicles from rat parotid gland.

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