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PMID: 3995563 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Isolation and partial characterization of two populations of secretory granules from rat parotid glands.

Cell and tissue research ·Vol. 240 ·No. 2 ·1985-00-00 ·Pages 441-7

Iversen JM, Kauffman DL, Keller PJ, Robinovitch M

Abstract

A method is described for the isolation of two populations of secretory granules from rat parotid glands utilizing differences in their sedimentation characteristics. The granule preparations were analyzed for homogeneity by electron microscopy and chemical analyses. The soluble contents of both types of granules were obtained by hypotonic lysis, and the proteins compared by SDS-PAGE and ion exchange-gel filtration chromatography. Both populations of secretory granules appear to have the same protein composition as that of the parotid saliva. The secretory granules with the smaller apparent buoyant density became labelled with radioactive leucine earlier than the heavier granules when a pulse of this amino acid was supplied to a gland slice system. The lighter granules appear to represent an earlier stage in maturation.

MeSH Terms
Animals Cell Fractionation Cytoplasmic Granules/analysis,ultrastructure Male Parotid Gland/analysis,ultrastructure Proteins/isolation & purification Rats Rats, Inbred Strains
Chemicals
Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Iversen J M
Kauffman D L
Keller P J
Robinovitch M
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29 references, click to expand
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Article Info
Journal
Cell and tissue research
Abbr.
Cell Tissue Res
ISSN
0302-766X
DOI
10.1007/BF00222357
Published
1985-00-00
Pages
441-7
Language
English
Region
Germany
NLM ID
0417625
Subset
IM
Grants
NIDCR NIH HHS · DE 02600 · United States
NIDCR NIH HHS · DE 05251 · United States
External Links
PubMed source DOI: 10.1007/BF00222357 View journal details
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