Multivalent regulation of isoleucine-valine transaminase in an Escherichia coli K-12 ilvA deletion strain.

Journal of bacteriology ·Vol. 130 ·No. 2 ·1977-05-00 ·Pages 951-3

Kline EL, Manross DN, Warwick ML

Abstract

In a strain of Escherichia coli K-12 lacking threonine deaminase, the enzyme converting alpha-ketoisovalerate and alpha-keto-beta-methylvalerate to valine and isoleucine, respectively, was multivalently repressed by valine, isoleucine, and leucine. This activity was due to transaminase B, specified by the ilvE structural gene.

MeSH Terms

Chromosome Deletion Escherichia coli/enzymology,genetics Genes Isoleucine/metabolism Threonine Dehydratase/genetics,physiology Transaminases/analysis,genetics,metabolism Valine/metabolism

Chemicals

Isoleucine Transaminases branched-chain-amino-acid transaminase Threonine Dehydratase Valine

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Kline E L

Manross D N

Warwick M L

References (9)

9 references, click to expand

REGULATORY MECHANISMS IN THE BIOSYNTHESIS OF ISOLEUCINE AND VALINE. II. IDENTIFICATION OF TWO OPERATOR GENES.
J Bacteriol. 1965 Mar;89:654-60 PMID: 14273640
Isoleucine and valine metabolism in Escherichia coli. V. alpha-Ketoisovaleric acid accumulation.
J Biol Chem. 1953 Nov;205(1):475-82 PMID: 13117924
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
Regulation of transaminase C synthesis in Escherichia coli: conditional leucine auxotrophy.
J Bacteriol. 1974 Nov;120(2):715-23 PMID: 4616947
The effect of an ilvA Mu phage insertion on ilv gene expression in Escherichia coli K-12.
Biochem Biophys Res Commun. 1975 Apr 21;63(4):1048-55 PMID: 1093546
Isoleucine and valine metabolism of Escherichia coli. XV. Biochemical properties of mutants resistant to thiaisoleucine.
J Bacteriol. 1968 May;95(5):1672-9 PMID: 4297021
The regulation of the ilv ADGE operon: evidence for positive control by threonine deaminase.
J Mol Biol. 1976 Apr 15;102(3):453-65 PMID: 775108
Regulation of isoleucine-valine biosynthesis in an ilvDAC deletion strain of Escherichia coli K-12.
Biochem Biophys Res Commun. 1974 Apr 23;57(4):1144-51 PMID: 4598336
An assay for transaminase B enzyme activity in Escherichia coli K-12.
Anal Biochem. 1973 Jan;51(1):67-79 PMID: 4569372

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.130.2.951-953.1977

Published

1977-05-00

Pages

951-3

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC235303

Subset

External Links

PubMed source DOI: 10.1128/jb.130.2.951-953.1977 PMC Full Text View journal details

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Multivalent regulation of isoleucine-valine transaminase in an Escherichia coli K-12 ilvA deletion strain.

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