Home LiteratureArticle Details
PMID: 4011432 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

DNA methylation of viruses infecting a eukaryotic Chlorella-like green alga.

Nucleic acids research ·Vol. 13 ·No. 10 ·1985-05-24 ·Pages 3471-8

Van Etten JL, Schuster AM, Girton L, Burbank DE, Swinton D, Hattman S

Abstract

The genomic DNAs of the eukaryotic Chlorella-like green alga, strain NC64A, and eleven of its viruses all contain significant levels of 5-methyldeoxycytidine. In addition, the host DNA as well as six of the viral DNAs also contain N6-methyldeoxyadenosine. At least some of the methylated bases in the host reside in different base sequences than the methylated bases in the viruses as shown by differential susceptibility to restriction endonuclease enzymes. This suggests that the viruses encode for DNA methyltransferases with sequence specificities different from that of the host enzyme.

MeSH Terms
Base Composition Base Sequence Chlorella/genetics DNA/metabolism DNA (Cytosine-5-)-Methyltransferases/analysis DNA, Viral/metabolism Methylation
Chemicals
DNA, Viral DNA DNA (Cytosine-5-)-Methyltransferases
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Van Etten J L
Schuster A M
Girton L
Burbank D E
Swinton D
Hattman S
References (18)
18 references, click to expand
  1. [The presence of 5-methylcytosine and 6-methylaminopurine in the composition of DNA in some algae].
    Dokl Akad Nauk SSSR. 1968 Sep 21;182(3):712-4 PMID: 4982456
  2. ( 6 N)methyl adenine in the nuclear DNA of a eucaryote, Tetrahymena pyriformis.
    J Cell Biol. 1973 Mar;56(3):697-701 PMID: 4631666
  3. Methylated bases in DNA from Paramecium aurelia.
    Biochim Biophys Acta. 1974 Nov 20;374(1):1-11 PMID: 4429738
  4. Hydroxymethyluracil in eukaryote DNA: a natural feature of the pyrrophyta (dinoflagellates).
    Science. 1976 Dec 3;194(4269):1062-4 PMID: 988637
  5. Comparative study of DNA methylation in three unicellular eucaryotes.
    J Bacteriol. 1978 Sep;135(3):1156-7 PMID: 99431
  6. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.
    Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683-7 PMID: 291033
  7. Molecular cloning, refined physical map and heterogeneity of methylation sites of papilloma virus type 1a DNA.
    Eur J Biochem. 1980 Aug;109(2):457-61 PMID: 6250842
  8. Frog virus 3 DNA is heavily methylated at CpG sequences.
    Virology. 1980 Nov;107(1):250-7 PMID: 6255678
  9. DNA methylation--a regulatory signal in eukaryotic gene expression.
    J Gen Virol. 1981 Nov;57(Pt 1):1-20 PMID: 6275010
  10. The effect of site specific methylation on restriction endonuclease cleavage (update).
    Nucleic Acids Res. 1983 Jan 11;11(1):r169-73 PMID: 6306558
  11. DNA methylation and gene activity.
    Annu Rev Biochem. 1983;52:93-124 PMID: 6311083
  12. Insertion mutations in the dam gene of Escherichia coli K-12.
    Mol Gen Genet. 1983;192(1-2):288-9 PMID: 6316110
  13. DNA methyltransferase induced by frog virus 3.
    J Virol. 1984 Jan;49(1):86-91 PMID: 6690723
  14. 5-Methylcytosine is not detectable in Saccharomyces cerevisiae DNA.
    Mol Cell Biol. 1984 May;4(5):985-8 PMID: 6374428
  15. Uneven distribution of methylation sites within the human papillomavirus la genome: possible relevance to viral gene expression.
    Nucleic Acids Res. 1984 Dec 11;12(23):8847-60 PMID: 6096809
  16. Lytic viruses infecting a Chlorella-like alga.
    Virology. 1985 Jan 15;140(1):135-43 PMID: 2981448
  17. Deoxyribonucleic acid methylation and chromatin organization in Tetrahymena thermophila.
    Mol Cell Biol. 1981 Jul;1(7):600-8 PMID: 9279374
  18. Occurrence of 5-methylcytosine in nucleic acids.
    Nature. 1950 Aug 5;166(4214):237-8 PMID: 15439258
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/13.10.3471
Published
1985-05-24
Pages
3471-8
Language
English
Region
England
NLM ID
0411011
PMCID
PMC341253
Subset
IM
Grants
NIGMS NIH HHS · GM-29227 · United States
NIGMS NIH HHS · GM-32441 · United States
External Links
PubMed source DOI: 10.1093/nar/13.10.3471 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2