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PMID: 4034395 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Evidence for sequence-specific conformational changes in DNA from the melting temperatures of DNA phosphorothioate derivatives.

Nucleic acids research ·Vol. 13 ·No. 15 ·1985-08-12 ·Pages 5707-16

Suggs JW, Taylor DA

Abstract

Analogs of alternating purine-pyrimidine DNA polymers such as poly(dA-dT)-poly(dA-dT) can be made with phosphorothioate groups in the DNA backbone. A phosphorothioate diester at the 5'-purine-pyrimidine-3' step causes a significant lowering of the polymer's melting temperature compared to a phosphorothioate diester at the 5'-pyrimidine-purine-3' step. This may occur because sulfur substitution increases anionic charge density in the DNA minor groove and 5'-purine-pyrimidine-3' steps narrow the minor groove. The ability to modulate charge density in the DNA backbone via sulfur substitution should prove useful in studies of sequence-dependent conformational changes in DNA.

MeSH Terms
Crystallization DNA Hot Temperature Magnetic Resonance Spectroscopy Nucleic Acid Conformation Nucleic Acid Heteroduplexes Organothiophosphates Organothiophosphorus Compounds Poly dA-dT/metabolism Pyrrolidines/metabolism Solutions Structure-Activity Relationship Thermodynamics
Chemicals
Nucleic Acid Heteroduplexes Organothiophosphates Organothiophosphorus Compounds Pyrrolidines Solutions Poly dA-dT dipyrandium DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Suggs J W
Taylor D A
References (25)
25 references, click to expand
  1. Nucleic acid-small molecule interactions. VII. Further characterization of deoxyribonucleic acid-diamino steroid complexes.
    Biochemistry. 1968 Apr;7(4):1568-82 PMID: 4878044
  2. Solution structure of poly(dA-dT).poly(dA-dT) in low and high salt: a 500 MHz 1H NMR study using one-dimensional NOE.
    J Biomol Struct Dyn. 1984 Jun;1(6):1423-55 PMID: 6400829
  3. Helix formation by dAT oligomers. I. Hairpin and straight-chain helices.
    J Mol Biol. 1968 Sep 28;36(3):291-304 PMID: 5760542
  4. The specificity of pancreatic deoxyribonuclease.
    Eur J Biochem. 1973 Dec 3;40(1):143-7 PMID: 4589546
  5. Organization of DNA in chromatin.
    Proc Natl Acad Sci U S A. 1976 Sep;73(9):3068-72 PMID: 1067602
  6. Physicochemical studies on the interaction of irehdiamine A with bihelical DNA.
    Biochemistry. 1977 Dec 27;16(26):5879-89 PMID: 588563
  7. Steroid diamine-nucleic acid interactions: partial insertion of dipyrandium between unstacked base pairs of the poly(dA-dT) duplex in solution.
    Proc Natl Acad Sci U S A. 1979 Jan;76(1):24-8 PMID: 284337
  8. An alternating conformation characterizes the phosphodiester backbone of poly(dA-dT) in solution.
    J Biol Chem. 1979 Sep 10;254(17):8125-8 PMID: 468810
  9. A hypothesis on a specific sequence-dependent conformation of DNA and its relation to the binding of the lac-repressor protein.
    J Mol Biol. 1979 Jul 15;131(4):669-80 PMID: 513130
  10. Nucleotide conformational analysis by 31P nuclear magnetic resonance spectroscopy.
    Annu Rev Biophys Bioeng. 1981;10:355-86 PMID: 7020579
  11. Helical periodicity of DNA, Poly(dA) . poly(dT) and poly(dA-dT). poly(dA-dT) in solution.
    Eur J Biochem. 1981 Aug;118(2):215-22 PMID: 7285918
  12. Sequence-dependent variation in the conformation of DNA.
    J Mol Biol. 1981 Jul 15;149(4):745-60 PMID: 6273590
  13. 13C NMR study of conformation and mobility of 145-base-pair poly(dA-dT) . poly(dA-dT) in solution.
    Eur J Biochem. 1981 Nov;120(2):309-12 PMID: 7318827
  14. The molecular structure of d(ICpCpGpG), a fragment of right-handed double helical A-DNA.
    Nature. 1982 Jan 28;295(5847):294-9 PMID: 7057893
  15. Reversible bending and helix geometry in a B-DNA dodecamer: CGCGAATTBrCGCG.
    J Biol Chem. 1982 Dec 25;257(24):14686-707 PMID: 7174662
  16. Mechanics of sequence-dependent stacking of bases in B-DNA.
    J Mol Biol. 1982 Oct 25;161(2):343-52 PMID: 7154084
  17. Wrinkled DNA.
    Nucleic Acids Res. 1983 Mar 11;11(5):1457-74 PMID: 6572358
  18. Salt-induced conformational transition of poly[d(A-T)] X poly[d(A-T)].
    J Mol Biol. 1983 May 5;166(1):85-92 PMID: 6854636
  19. Sequence-dependent conformation of an A-DNA double helix. The crystal structure of the octamer d(G-G-T-A-T-A-C-C).
    J Mol Biol. 1983 May 15;166(2):183-201 PMID: 6854642
  20. Generation of left-handed Z-DNA in solution and visualization in polytene chromosomes by immunofluorescence.
    Cold Spring Harb Symp Quant Biol. 1983;47 Pt 1:143-54 PMID: 6345056
  21. Right-handed and left-handed double-helical DNA: structural studies.
    Cold Spring Harb Symp Quant Biol. 1983;47 Pt 1:33-44 PMID: 6574849
  22. Assignment of resonances in the phosphorus-31 nuclear magnetic resonance spectrum of poly[d(A-T)] from phosphorothioate substitution.
    Biochemistry. 1983 Sep 13;22(19):4546-50 PMID: 6354262
  23. Poly(dA-dT) has a right-handed B conformation in solution: a two-dimensional NMR study.
    Biochemistry. 1984 Feb 28;23(5):791-6 PMID: 6712923
  24. DNA structural variations in the E. coli tyrT promoter.
    Cell. 1984 Jun;37(2):491-502 PMID: 6327070
  25. Polyribonucleotides containing a phosphorothioate backbone.
    Eur J Biochem. 1970 Apr;13(3):558-64 PMID: 5444162
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/13.15.5707
Published
1985-08-12
Pages
5707-16
Language
English
Region
England
NLM ID
0411011
PMCID
PMC321900
Subset
IM
Grants
NCI NIH HHS · CA00947 · United States
NIGMS NIH HHS · GM 31586 · United States
External Links
PubMed source DOI: 10.1093/nar/13.15.5707 PMC Full Text View journal details
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