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PMID: 4038273 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Inositol phosphate formation in fMet-Leu-Phe-stimulated human neutrophils does not require an increase in the cytosolic free Ca2+ concentration.

The Biochemical journal ·Vol. 229 ·No. 2 ·1985-07-15 ·Pages 361-7

Di Virgilio F, Vicentini LM, Treves S, Riz G, Pozzan T

Abstract

The accumulation of inositol phosphates in myo-[3H]inositol-labelled human neutrophils stimulated with the chemotactic peptide fMet-Leu-Phe was measured. The challenge with the chemotactic peptide caused the generation of inositol monophosphate (InsP), inositol bisphosphate (InsP2) and inositol trisphosphate (InsP3). The formation of the three inositol phosphates followed a differential time course: InsP3 accumulated very rapidly and transiently, whereas InsP increased steadily for more than 2 min. Inositol phosphate formation was only partially decreased by procedures which prevented the fMet-Leu-Phe-dependent increase of cytosolic free Ca2+ concentration.

MeSH Terms
Calcium/metabolism Cytoplasm/metabolism Cytosol/metabolism Humans Inositol Phosphates/blood N-Formylmethionine Leucyl-Phenylalanine/pharmacology Neutrophils/metabolism Oxygen Consumption Sugar Phosphates/blood
Chemicals
Inositol Phosphates Sugar Phosphates inositol 1-phosphate N-Formylmethionine Leucyl-Phenylalanine Calcium
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Di Virgilio F
Vicentini L M
Treves S
Riz G
Pozzan T
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31 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2290361
Published
1985-07-15
Pages
361-7
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1145068
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2290361 PMC Full Text View journal details
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