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PMID: 4040519 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Intracellular transport of phosphatidylcholine to the plasma membrane.

The Journal of cell biology ·Vol. 101 ·No. 2 ·1985-08-00 ·Pages 441-5

Kaplan MR, Simoni RD

Abstract

We have used pulse-chase labeling of Chinese hamster ovary cells with choline followed by plasma membrane isolation on cationic beads to study the transport of phosphatidylcholine from the endoplasmic reticulum to the plasma membrane. We have found that the process is rapid (t1/2 [25 degrees C] = 2 min) and not affected by energy poisons or by cytochalasin B, colchicine, monensin, or carbonyl cyanide p-chlorophenylhydrazone. Cooling cells to 0 degree C effectively stops the transport process. The intracellular transport of phosphatidylcholine is distinct in several ways from the intracellular transport of cholesterol (Kaplan, M. R., and R. D. Simoni, 1985, J. Cell. Biol., 101:446-453).

MeSH Terms
Animals Biological Transport, Active/drug effects Carbonyl Cyanide m-Chlorophenyl Hydrazone/pharmacology Cell Line Cell Membrane/metabolism Choline/metabolism Colchicine/pharmacology Cricetinae Cricetulus Cytochalasin B/pharmacology Endoplasmic Reticulum/metabolism Female Fluorides Kinetics Monensin/pharmacology Ovary Phosphatidylcholines/metabolism Potassium/pharmacology Potassium Compounds Potassium Cyanide/pharmacology Temperature
Chemicals
Phosphatidylcholines Potassium Compounds Cytochalasin B Carbonyl Cyanide m-Chlorophenyl Hydrazone Monensin potassium fluoride Potassium Cyanide Choline Fluorides Potassium Colchicine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kaplan M R
Simoni R D
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23 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.101.2.441
Published
1985-08-00
Pages
441-5
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2113683
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.101.2.441 PMC Full Text View journal details
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