Intramolecular heterogeneity of mitochondrial DNA of Drosophila melanogaster.

The Journal of cell biology ·Vol. 73 ·No. 2 ·1977-05-00 ·Pages 279-86

Goldring ES, Peacock WJ

Abstract

DNA from purified mitochondria of Drosophila melanogaster can be isolated as supercoiled molecules which when nicked have a contour length of 5.9 micron. Partial denaturation mapping shows regional heterogeneity of base composition with one early denaturing region, with a calculated GC content close to zero, extending over 20% of the genome. DNA isolated from unfertilized eggs shows nuclear and mitochondrial DNA in equal proportions; we found no evidence of other cytoplasmic species.

MeSH Terms

Animals Cytosine/analysis DNA/analysis DNA, Mitochondrial/analysis Drosophila melanogaster/analysis Female Guanine/analysis Nucleic Acid Conformation Nucleic Acid Denaturation Nucleic Acid Renaturation Ovum/analysis

Chemicals

DNA, Mitochondrial Guanine Cytosine DNA

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Goldring E S

Peacock W J

References (13)

13 references, click to expand

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Article Info

Journal

The Journal of cell biology

Abbr.

J Cell Biol

ISSN

0021-9525

DOI

10.1083/jcb.73.2.279

Published

1977-05-00

Pages

279-86

Language

English

Region

United States

NLM ID

0375356

PMCID

PMC2109910

Subset

External Links

PubMed source DOI: 10.1083/jcb.73.2.279 PMC Full Text View journal details

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Intramolecular heterogeneity of mitochondrial DNA of Drosophila melanogaster.

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