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PMID: 4044037 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Monoclonal antibodies against surface antigens of Bacteroides gingivalis.

Infection and immunity ·Vol. 50 ·No. 1 ·1985-10-00 ·Pages 231-5

Naito Y, Okuda K, Kato T, Takazoe I

Abstract

Monoclonal antibodies against the various surface antigens of Bacteroides gingivalis were obtained by the fusion of murine myeloma cells (SP2/0-Ag14) with spleen cells of BALB/c mice immunized with the whole cells. Two monoclonal antibodies reacted with lipopolysaccharide, and the other two reacted strongly with capsule antigen. One showed reactivity with the hemagglutinin of the cells. The five monoclonal antibodies reacted with sonicated antigen from all B. gingivalis strains tested. No cross-reactivity of the monoclonal antibodies with antigens from nine species of other black-pigmented Bacteroids strains was observed. An immunoblotting test involving the use of these monoclonal antibodies indicated that the epitope of B. gingivalis lipopolysaccharide was polysaccharide with a high molecular weight of 40,000 to 60,000. The immunoblotting test also demonstrated that the epitopes of capsule antigen and of hemagglutinin were 27,000- and 40,000-molecular-weight proteins, respectively.

MeSH Terms
Antibodies, Bacterial/immunology Antibodies, Monoclonal/immunology Antibody Specificity Antigens, Bacterial/immunology Antigens, Surface/immunology Bacterial Proteins/immunology Bacteroides/immunology Hemagglutinins/immunology Lipopolysaccharides/immunology Molecular Weight Polysaccharides, Bacterial/immunology
Chemicals
Antibodies, Bacterial Antibodies, Monoclonal Antigens, Bacterial Antigens, Surface Bacterial Proteins Hemagglutinins Lipopolysaccharides Polysaccharides, Bacterial
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Naito Y
Okuda K
Kato T
Takazoe I
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20 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.50.1.231-235.1985
Published
1985-10-00
Pages
231-5
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC262161
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.50.1.231-235.1985 PMC Full Text View journal details
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