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PMID: 40504910 Published · ppublish English Journal Article

Rapid model-guided design of organ-scale synthetic vasculature for biomanufacturing.

Science (New York, N.Y.) ·Vol. 388 ·No. 6752 ·2025-06-12 ·页码 1198-1204

Sexton ZA, Rütsche D, Herrmann JE, Hudson AR, Sinha S, Du J, Shiwarski DJ, Masaltseva A, Solberg FS, Pham J, Szafron JM, Wu SM, Feinberg AW, Skylar-Scott MA, Marsden AL

Abstract

Our ability to produce human-scale biomanufactured organs is limited by inadequate vascularization and perfusion. For arbitrarily complex geometries, designing and printing vasculature capable of adequate perfusion poses a major hurdle. We introduce a model-driven design platform that demonstrates rapid synthetic vascular model generation alongside multifidelity computational fluid dynamics simulations and three-dimensional bioprinting. Key algorithmic advances accelerate vascular generation 230-fold and enable application to arbitrarily complex shapes. We demonstrate that organ-scale vascular network models can be generated and used to computationally vascularize >200 engineered and anatomic models. Synthetic vascular perfusion improves cell viability in fabricated living-tissue constructs. This platform enables the rapid, scalable vascular model generation and fluid physics analysis for biomanufactured tissues that are necessary for future scale-up and production.

MeSH 主题词

Tissue Engineering/methods Humans Bioprinting/methods Printing, Three-Dimensional Blood Vessels/anatomy & histology Algorithms Hydrodynamics Cell Survival Models, Anatomic Human Umbilical Vein Endothelial Cells Neovascularization, Physiologic Perfusion

作者与单位

共 15 位作者，点击展开单位 / ORCID

Sexton Zachary A ORCID

Department of Bioengineering, Stanford University, Stanford, CA, USA. | Cardiovascular Institute, Stanford University, Stanford, CA, USA.

Rütsche Dominic ORCID

Department of Bioengineering, Stanford University, Stanford, CA, USA. | Cardiovascular Institute, Stanford University, Stanford, CA, USA.

Herrmann Jessica E ORCID

School of Medicine, Stanford University, Stanford, CA, USA.

Hudson Andrew R ORCID

Department of Biomedical Engineering, Carnegie Mellon University, Pittsburgh, PA, USA.

Sinha Soham

Department of Bioengineering, Stanford University, Stanford, CA, USA.

Du Jianyi ORCID

Department of Bioengineering, Stanford University, Stanford, CA, USA.

Shiwarski Daniel J ORCID

Department of Biomedical Engineering, Carnegie Mellon University, Pittsburgh, PA, USA.

Masaltseva Anastasiia ORCID

Department of Bioengineering, Stanford University, Stanford, CA, USA.

Solberg Fredrik Samdal

Department of Mechanical Engineering, Stanford University, Stanford, CA, USA.

Pham Jonathan ORCID

Department of Mechanical Engineering, Stanford University, Stanford, CA, USA.

Szafron Jason M ORCID

Cardiovascular Institute, Stanford University, Stanford, CA, USA. | Department of Pediatrics, Stanford University, Stanford, CA, USA.

Wu Sean M ORCID

Cardiovascular Institute, Stanford University, Stanford, CA, USA. | Division of Cardiovascular Medicine, Department of Medicine, Stanford University, Stanford, CA, USA.

Feinberg Adam W ORCID

Department of Biomedical Engineering, Carnegie Mellon University, Pittsburgh, PA, USA. | Department of Materials Science and Engineering, Carnegie Mellon University, Pittsburgh, PA, USA.

Skylar-Scott Mark A ORCID

Department of Bioengineering, Stanford University, Stanford, CA, USA. | Cardiovascular Institute, Stanford University, Stanford, CA, USA. | Basic Science and Engineering Initiative, Children's Heart Center, Stanford University, Stanford, CA, USA. | Chan Zuckerberg Biohub, San Francisco, CA, USA.

Marsden Alison L ORCID

Department of Bioengineering, Stanford University, Stanford, CA, USA. | Cardiovascular Institute, Stanford University, Stanford, CA, USA. | Department of Pediatrics, Stanford University, Stanford, CA, USA. | Institute of Computational and Mathematical Engineering, Stanford University, Stanford, CA, USA.

Article Info

Journal

Science (New York, N.Y.)

Abbr.

Science

ISSN

1095-9203

DOI

10.1126/science.adj6152

Published

2025-06-12

电子出版

2025-00-12

页码

1198-1204

Language

English

Country/Region

United States

NLM ID

0404511

基金资助

NHLBI NIH HHS · T32 HL166155 · United States

NIBIB NIH HHS · R01 EB029362 · United States

NHLBI NIH HHS · DP2 HL168563 · United States

NHLBI NIH HHS · K99 HL155777 · United States

NHLBI NIH HHS · R01 HL141712 · United States

勘误 / 撤稿关联

UpdateOf

PMID: 37645046

CommentIn

PMID: 40504928

External Links

PubMed source DOI: 10.1126/science.adj6152 View journal details

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Rapid model-guided design of organ-scale synthetic vasculature for biomanufacturing.

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