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PMID: 405498 Published · ppublish English Journal Article

An analysis of the binding of fluorescence probes in mitochondrial systems.

The Journal of membrane biology ·Vol. 33 ·No. 1-2 ·1977-05-06 ·Pages 21-40

Williams WP, Layton DG, Johnston C

Abstract

Measurements of the binding of the fluorescent probes 8-anilinonaphthalene-1-sulfonate (ANS) and ethidium ions to whole and disruped mitochondria and submitochondrial particles suggest that the inner mitochondrial membrane is freely permeable to the two probes. Equations relating the binding of permeant probes to the electro-chemical balance across the membrane of vesicular systems are derived and these equations used to analyze Scatchard plots of the binding of the two probes to energized and nonenergized mitochondria and EDTA particles.

MeSH Terms
Anilino Naphthalenesulfonates/metabolism,pharmacology Animals Binding Sites Cattle Cell Fractionation Edetic Acid/pharmacology Ethidium/pharmacology Kinetics Mathematics Membranes/drug effects,metabolism,ultrastructure Mitochondria, Muscle/drug effects,metabolism,ultrastructure Myocardium Oxidative Phosphorylation/drug effects Oxygen Consumption/drug effects Sonication
Chemicals
Anilino Naphthalenesulfonates Edetic Acid Ethidium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Williams W P
Layton D G
Johnston C
References (20)
20 references, click to expand
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Article Info
Journal
The Journal of membrane biology
Abbr.
J Membr Biol
ISSN
0022-2631
DOI
10.1007/BF01869510
Published
1977-05-06
Pages
21-40
Language
English
Region
United States
NLM ID
0211301
Subset
IM
External Links
PubMed source DOI: 10.1007/BF01869510 View journal details
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