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PMID: 405969 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Mechanism of suppression in Drosophila. V. Localization of the purple mutant of Drosophila melanogaster in the pteridine biosynthetic pathway.

Biochemical genetics ·Vol. 15 ·No. 3-4 ·1977-04-00 ·Pages 321-32

Wilson TG, Jacobson KB

Abstract

The suppressible eye color mutant purple (pr) of Drosophila melanogaster is known to be unable to synthesize a wild-type complement of pteridine eye pigments. This study measures the reduced levels of drosopterins, sepiapterin, and an unidentified presumed pteridine in pr and prbw. Pteridine analyses in double mutants combining pr with one of three other eye color mutants sepia, Henna-recessive3, and prune2, suggest that the metabolic block in pr occurs prior to sepiapterin biosynthesis. Measurements of GTP and GTP cyclohydrolase in pr showed wild-type levels and indicate the metabolic block in pr to be at one of the steps converting dihydroneopterin triphosphate to sepiapterin. Quantitation of pteridines in suppressed purple [su(s)2; pr and pr; su(pr)e3] shows restoration of pteridines to wild-type or nearly wild-type levels.

MeSH Terms
Aging Animals Drosophila melanogaster/growth & development,metabolism Eye Female GTP Cyclohydrolase/metabolism Guanosine Triphosphate/metabolism Male Mutation Pteridines/metabolism Recombination, Genetic Species Specificity Spectrometry, Fluorescence
Chemicals
Pteridines Guanosine Triphosphate GTP Cyclohydrolase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wilson T G
Jacobson K B
References (9)
9 references, click to expand
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Article Info
Journal
Biochemical genetics
Abbr.
Biochem Genet
ISSN
0006-2928
DOI
10.1007/BF00484463
Published
1977-04-00
Pages
321-32
Language
English
Region
United States
NLM ID
0126611
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00484463 View journal details
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