Draft genome sequence and annotation of the enfumafungin producing fungus Hormonema carpetanum ATCC 74360.

Plant endophytic fungusHormonema carpetanum is the only organism known to produce enfumafungin to date. Enfumafungin is a fernane-type triterpenoid glycoside compound with broad-spectrum antifungal activity, and this leading compound has been developed to antifungal agent ibrexafungerp approved by Food and Drug Administration (FDA). Here we report the first de novo assembled and annotated whole-genome sequence of H. carpetanum ATCC 74360, the original enfumafungin producing strain from the American Type Culture Collection (ATCC). We sequenced the genome of H. carpetanum ATCC 74360 through a hybrid sequencing strategy combining Illumina NovaSeq X Plus short-read and PacBio Sequel IIe long-read technologies. Data from the two platforms were combined and the de novo assembly estimated a 32.93 Mbp genome divided into 16 contigs with a GC content of 49.85%. Genome annotation identified 10,433 genes, 253 tRNAs, and 62 rRNAs. antiSMASH predicted biosynthesis gene cluster (BGC) of secondary metabolite, including enfumafungin BGC. This genome and related data files presented here will provide a foundation for further gene editing of this important antibiotic-producing microorganism.