SPARC Induces COL1A1/COL3A1 Expressions Representing Aggressive Molecular Cancer-Associated Fibroblasts Signatures and CSF1-Mediated Cancer Invasion in Colorectal Cancer.

Secreted protein, acidic and rich in cysteine (SPARC) may play an important role in tumor microenvironment (TME) contribution to metastasis, however molecular mechanism of SPARC contribution to TME remains elusive. In this study, SPARC molecular status was explored for its clinical and functional relevance to metastasis in the context of its stromal overexpression, which may represent colorectal cancer (CRC) progression. SPARC expression was highly specific to cancer stroma of CRC tumors (GSE35608) and exhibited prognostic relevance. SPARC knockdown (KD) in cancer-associated fibroblasts (CAFs) resulted in diminished metastatic phenotypes, accompanied by robust suppression of COL1A1/COL3A1 expressions and multiple secretome genes. Among the secretome genes, CSF1 was closely associated with stromal SPARC expression, and it was demonstrated that the CSF1/CSF1R axis was required for SPARC-induced cancer invasion. Comprehensive selection by stromal specificity and its SPARC association identified CAF-associated genes (CAFGs), including classical CAFs markers and COL family genes, which were uniquely enriched as higher priority for prognosis. These findings suggest that SPARC-mediated induction of COL family genes and secretome in the TME may be primarily featured against the host rather than be protective. Our present findings may aid in simplifying molecular understanding of aggressive TME and enrich bona-fide therapeutic targets for cancer metastasis.