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PMID: 4084229 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Rapid formation of inositol 1,3,4,5-tetrakisphosphate following muscarinic receptor stimulation of rat cerebral cortical slices.

The Biochemical journal ·Vol. 232 ·No. 1 ·1985-11-15 ·Pages 211-5

Batty IR, Nahorski SR, Irvine RF

Abstract

Carbachol stimulation of muscarinic receptors in rat cortical slices prelabelled with myo-[2-3H]inositol caused the rapid formation of a novel inositol polyphosphate. Evidence derived from its chromatographic behaviour, and from the structure of the products formed in partial dephosphorylation experiments, suggests that it is probably D-myo-inositol 1,3,4,5-tetrakisphosphate. An enzyme in human red cell membranes specifically removes the 5-phosphate from it to form inositol 1,3,4-trisphosphate. It is suggested that inositol 1,3,4,5-tetrakisphosphate is likely to be a second messenger, and that it is the precursor of inositol 1,3,4-trisphosphate and possibly of inositol 1,4,5-trisphosphate.

MeSH Terms
Animals Carbachol/pharmacology Cerebral Cortex/drug effects,metabolism In Vitro Techniques Inositol Phosphates/metabolism,pharmacology Male Rats Rats, Inbred Strains Receptors, Muscarinic/drug effects Stimulation, Chemical Sugar Phosphates/metabolism
Chemicals
Inositol Phosphates Receptors, Muscarinic Sugar Phosphates inositol-1,3,4,5-tetrakisphosphate Carbachol
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Batty I R
Nahorski S R
Irvine R F
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25 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2320211
Published
1985-11-15
Pages
211-5
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1152860
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2320211 PMC Full Text View journal details
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