Isolated hapten-binding receptors of sensitized lymphocytes. II. Receptors from nylon wool-enriched rabbit T lymphocytes lack serological determinants of immunoglobulin constant domains but carry the A locus allotypic markers.

The Journal of experimental medicine ·Vol. 146 ·No. 3 ·1977-09-01 ·Pages 792-801

Krawinkel U, Cramer M, Mage RG, Kelus AS, Rajewsky K

Abstract

Hapten-binding receptor material was isolated from sensitized rabbit lymphocytes by a method described previously for murine receptor material. The material was separated into a fraction expressing immunoglobulin determinants (anti-Ig+ fraction) and a fraction lacking known class and type-specific determinants of Ig constant domains (anti-Ig- fraction). We present evidence in support of the notion that--in analogy to the mouse system--the anti-Ig+ fraction is B-cell-derived, whereas the anti-Ig- fraction originates from T lymphocytes. Receptors of the anti-Ig- phenotype are found to express a locus allotypic determinants and, thus, appear to carry variable portions of immunoglobulin heavy chains.

MeSH Terms

Animals B-Lymphocytes/immunology Binding Sites, Antibody Genes Haptens Immunoglobulin A Immunoglobulin Fc Fragments Immunoglobulin G Immunoglobulin Heavy Chains Immunoglobulin M Phenotype Rabbits T-Lymphocytes/immunology

Chemicals

Haptens Immunoglobulin A Immunoglobulin Fc Fragments Immunoglobulin G Immunoglobulin Heavy Chains Immunoglobulin M

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Krawinkel U

Cramer M

Mage R G

Kelus A S

Rajewsky K

References (21)

21 references, click to expand

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Article Info

Journal

The Journal of experimental medicine

Abbr.

J Exp Med

ISSN

0022-1007

DOI

10.1084/jem.146.3.792

Published

1977-09-01

Pages

792-801

Language

English

Region

United States

NLM ID

2985109R

PMCID

PMC2180802

Subset

External Links

PubMed source DOI: 10.1084/jem.146.3.792 PMC Full Text View journal details

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Isolated hapten-binding receptors of sensitized lymphocytes. II. Receptors from nylon wool-enriched rabbit T lymphocytes lack serological determinants of immunoglobulin constant domains but carry the A locus allotypic markers.

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