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PMID: 4092678 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Biosynthesis and membrane topography of the neural cell adhesion molecule L1.

The EMBO journal ·Vol. 4 ·No. 12 ·1985-12-01 ·Pages 3105-13

Faissner A, Teplow DB, Kübler D, Keilhauer G, Kinzel V, Schachner M

Abstract

The biosynthesis and membrane topography of the neural cell adhesion molecule L1 have been studied in cerebellar cell cultures by metabolic labeling and immunoprecipitation. Pulse and pulse-chase experiments with [35S]methionine show that L1 is synthesized in its high mol. wt. form, the 200 kd component. The lower mol. wt. components with 40, 80 and 140 K apparent mol. wts. can be generated by proteolysis in intact cellular membranes. Peptide maps generated by protease treatment of L1 isolated from adult mouse brain show that the 80 and 140 kd components are related to the 200 kd component, but not to each other. The 200, 80 and 40 kd components can be biosynthetically phosphorylated. The 140 kd component is not phosphorylated and not released from the surface membrane during tryspinization. The phosphorylated amino acid is serine. In the presence of tunicamycin the 200 kd component is synthesized as a 150 kd protein. Pulse-chase experiments in the presence of tunicamycin indicate that the carbohydrate moieties are predominantly N-glycosidically linked and that the contribution of O-glycosylation is minimal. The carbohydrate moieties are of the complex type as shown by treatment with endoglycosidase H. Since monensin inhibits processing of the carbohydrate moieties, the 200 kd component appears to be transported to the surface membrane via the Golgi apparatus.

MeSH Terms
Amino Acids/analysis Animals Animals, Newborn Antigens, Surface/analysis,genetics Cell Adhesion Molecules Cell Membrane Permeability Cells, Cultured Cerebellum/embryology,metabolism Female Fucose/metabolism Glucosamine/metabolism Methionine/metabolism Mice Mice, Inbred C57BL Peptide Fragments/analysis Phosphates/metabolism Phosphorus Radioisotopes Pregnancy Sulfur Radioisotopes Tritium Trypsin
Chemicals
Amino Acids Antigens, Surface Cell Adhesion Molecules Peptide Fragments Phosphates Phosphorus Radioisotopes Sulfur Radioisotopes Tritium Fucose Methionine Trypsin Glucosamine
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Faissner A
Teplow D B
Kübler D
Keilhauer G
Kinzel V
Schachner M
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39 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1985-12-01
Pages
3105-13
Language
English
Region
England
NLM ID
8208664
PMCID
PMC554629
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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