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PMID: 4092685 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Developmental regulation of a gastrula-specific gene injected into fertilized Xenopus eggs.

The EMBO journal ·Vol. 4 ·No. 13A ·1985-12-16 ·Pages 3463-71

Krieg PA, Melton DA

Abstract

To study the transcriptional regulation of genes during early Xenopus development, we have isolated a gene that is first transcribed at the mid-blastula transition. Transcription of this gene, called GS17, stops at mid-gastrula and the mRNA is rapidly degraded. Consequently, transcripts of GS17 are only present for a brief period, primarily during gastrulation. When the GS17 gene is injected into fertilized eggs, transcription from the injected DNA mimics the expression pattern of the endogenous gene, i.e., both the switch-on and switch-off of transcription are correctly regulated. The injected DNA is not significantly amplified and remains extrachromosomal. The correct expression of genes injected into Xenopus eggs will make it possible to investigate maternal factors involved in activating the embryonic genome.

MeSH Terms
Animals Base Sequence Cloning, Molecular DNA, Recombinant Fibronectins/genetics Gastrula/physiology Gene Expression Regulation Globins/genetics Transcription, Genetic Xenopus laevis/embryology,genetics Zygote/physiology
Chemicals
DNA, Recombinant Fibronectins Globins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Krieg P A
Melton D A
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33 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1985-12-16
Pages
3463-71
Language
English
Region
England
NLM ID
8208664
PMCID
PMC554685
Subset
IM
Databases
GENBANK
X05215
External Links
PubMed source PMC Full Text View journal details
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