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PMID: 410018 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

DNA-directed synthesis in vitro of beta-galactosidase: requirement for a ribosome release factor.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 74 ·No. 8 ·1977-08-00 ·Pages 3217-21

Kung HF, Treadwell BV, Spears C, Tai PC, Weissbach H

Abstract

The DNA-directed synthesis of beta-galactosidase in Escherichia coli extracts has been investigated in a partially fractionated system. A dependency was obtained for 3',5'-cyclic AMP receptor protein and also for a factor, from the salt wash of ribosomes, that has been purified to near homogeneity. This factor has been identified with a ribosome release factor previously purified from the supernatant fraction by A. Hirashima and A. Kaji [(1972) Biochemistry 11,4037-4044]. In the coupled transcription-translation system this factor stimulates beta-galactosidase synthesis and total protein synthesis 2- to 4-fold. It is thus clear that the ribosome release factor has a physiological function in translation. It may also affect transcription, because it stimulated total RNA synthesis up to 50% in this in vitro system.

MeSH Terms
Cross Reactions DNA, Bacterial/metabolism Escherichia coli/enzymology Galactosidases/biosynthesis Immunodiffusion Kinetics Molecular Weight Peptide Chain Termination, Translational Polyribosomes/metabolism Protein Biosynthesis RNA, Messenger/metabolism Receptors, Cyclic AMP/metabolism Ribosomal Proteins/isolation & purification,metabolism Transcription, Genetic beta-Galactosidase/biosynthesis
Chemicals
DNA, Bacterial RNA, Messenger Receptors, Cyclic AMP Ribosomal Proteins Galactosidases beta-Galactosidase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Kung H F
Treadwell B V
Spears C
Tai P C
Weissbach H
References (30)
30 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.74.8.3217
Published
1977-08-00
Pages
3217-21
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC431503
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.74.8.3217 PMC Full Text View journal details
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