IgE and IgGa antibody-mediated release of histamine from rat peritoneal cells. I. Optimum conditions for in vitro preparation of target cells with antibody and and challenge with antigen.

The Journal of experimental medicine ·Vol. 133 ·No. 4 ·1971-04-01 ·Pages 752-71

Bach MK, Bloch KJ, Austen KF

Abstract

The optimum conditions for antigen-induced release of histamine in the rat IgE and IgGa antibody-mediated systems were studied in vitro. The IgE antibody-mediated reaction could be separated into two steps: preparation of target cells with antibody and challenge with antigen. The optimal conditions for these two steps were distinctly different. Release of histamine by IgGa antibody and antigen could not be separated into two steps, and the optimal conditions for the total reaction were identical to those of the antigen challenge step of the IgE antibody-mediated system.

MeSH Terms

Animals Antibodies Antigen-Antibody Reactions Calcium/pharmacology Heparin/pharmacology Histamine Release Hydrogen-Ion Concentration Immunity, Cellular Immunoglobulin G/physiology Immunoglobulins/physiology In Vitro Techniques Magnesium/pharmacology Male Mast Cells/immunology Osmolar Concentration Peritoneum/cytology,immunology Rats Urea/pharmacology

Chemicals

Antibodies Immunoglobulin G Immunoglobulins Urea Heparin Magnesium Calcium

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Bach M K

Bloch K J

Austen K F

References (27)

27 references, click to expand

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Article Info

Journal

The Journal of experimental medicine

Abbr.

J Exp Med

ISSN

0022-1007

DOI

10.1084/jem.133.4.752

Published

1971-04-01

Pages

752-71

Language

English

Region

United States

NLM ID

2985109R

PMCID

PMC2138970

Subset

External Links

PubMed source DOI: 10.1084/jem.133.4.752 PMC Full Text View journal details

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IgE and IgGa antibody-mediated release of histamine from rat peritoneal cells. I. Optimum conditions for in vitro preparation of target cells with antibody and and challenge with antigen.

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