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PMID: 4105039 Published · ppublish English Journal Article

The fine structural localization of endogenous and exogenous peroxidase activity in Kupffer cells of rat liver.

The Journal of cell biology ·Vol. 47 ·No. 1 ·1970-10-00 ·Pages 247-62

Fahimi HD

Abstract

Endogenous peroxidase activity has been demonstrated in sections of rat liver fixed briefly by glutaraldehyde perfusion and incubated in Graham and Karnovsky's medium for cytochemical demonstration of peroxidase activity (29). In 25-40% of sinusoidal cells, an electron-opaque reaction product is localized in segments of the endoplasmic reticulum, including the perinuclear cisternae, a few Golgi vesicles and saccules and in some large membrane-bounded granules. This staining is abolished after prolonged fixation or boiling of tissue sections in glutaraldehyde, and in the absence of H(2)O(2) or DAB from the incubation medium. Furthermore, the reaction is inhibited completely by sodium azide and high concentrations of H(2)O(2), and partially by KCN and aminotriazole. Among the different cells in hepatic sinusoids, the nonphagocytic "fat-storing" cells (39) are always peroxidase negative, whereas the lining cells in process of erythrophagocytosis are consistently peroxidase positive. The possible biological significance of endogenous peroxidase in Kupffer cells is discussed. In addition, the uptake of exogenous horseradish peroxidase by Kupffer cells has been investigated. The exogenous tracer protein, which in contrast to endogenous peroxidase of Kupffer cells is not inhibited by prolonged aldehyde fixation, is taken up by micropinocytosis and remains confined to the lysosomal system of Kupffer cells. The significance of these observations in respect to some recent studies suggesting localization of exogenous peroxidases in the endoplasmic reticulum of Kupffer cells and peritoneal macrophages (22, 23) is briefly discussed.

MeSH Terms
Aldehydes Animals Azides/pharmacology Cyanides/pharmacology Cytoplasmic Granules/enzymology Endoplasmic Reticulum/enzymology Golgi Apparatus/enzymology Histocytochemistry Hydrogen Peroxide Inclusion Bodies/enzymology Liver/cytology,enzymology Lysosomes/enzymology Male Microscopy, Electron Peroxidases/analysis,anatomy & histology,metabolism Phagocytosis Pinocytosis Rats Staining and Labeling Triazoles/pharmacology p-Dimethylaminoazobenzene
Chemicals
Aldehydes Azides Cyanides Triazoles p-Dimethylaminoazobenzene Hydrogen Peroxide Peroxidases
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Fahimi H D
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55 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.47.1.247
Published
1970-10-00
Pages
247-62
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2108393
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.47.1.247 PMC Full Text View journal details
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