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PMID: 4108111 Published · ppublish English Journal Article

Products of RNA polymerases in HeLa cell nuclei.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 68 ·No. 11 ·1971-11-00 ·Pages 2861-5

Zylber EA, Penman S

Abstract

RNA polymerase activities in intact HeLa cell nuclei have been examined and compared to activities investigated in previous studies of the purified enzymes. The RNA synthesized by the mammalian polymerases while still in nuclei is identified. The polymerases are tentatively identified by location, sensitivity to alpha-amanitin, and response to manganese and altered ionic strength. Polymerase I is located in the nucleolus and labels partially complete precursor molecules of ribosomal RNA. Polymerases II and III are in the nucleoplasm and both label giant nuclear heterogeneous RNA.

MeSH Terms
Cell Nucleus/drug effects,enzymology,metabolism Centrifugation, Density Gradient Cytosine Nucleotides/metabolism Dactinomycin/pharmacology Depression, Chemical HeLa Cells/enzymology,metabolism Humans Hydrogen-Ion Concentration Magnesium/pharmacology Manganese/pharmacology Mycotoxins/pharmacology Osmolar Concentration RNA Nucleotidyltransferases/metabolism Tritium Uracil Nucleotides/metabolism
Chemicals
Cytosine Nucleotides Mycotoxins Uracil Nucleotides Tritium Dactinomycin Manganese RNA Nucleotidyltransferases Magnesium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Zylber E A
Penman S
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24 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.68.11.2861
Published
1971-11-00
Pages
2861-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC389543
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.68.11.2861 PMC Full Text View journal details
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