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PMID: 4108501 Published · ppublish English Journal Article

Amino acid sequence of the encephalitogenic basic protein from human myelin.

The Biochemical journal ·Vol. 123 ·No. 1 ·1971-06-00 ·Pages 57-67

Carnegie PR

Abstract

Myelin from the central nervous system contains an unusual basic protein, which can induce experimental autoimmune encephalomyelitis. The basic protein from human brain was digested with trypsin and other enzymes and the sequence of the 170 amino acids was determined. The localization of the encephalitogenic determinants was described. Possible roles for the protein in the structure and function of myelin are discussed.

MeSH Terms
Amino Acid Sequence Chromatography, Gel Chromatography, Paper Cyanogen Bromide Electrophoresis Encephalomyelitis, Autoimmune, Experimental/etiology Epitopes Humans Myelin Sheath/analysis Nerve Tissue Proteins/analysis Pepsin A Peptides/analysis Thermolysin Trypsin
Chemicals
Epitopes Nerve Tissue Proteins Peptides Trypsin Pepsin A Thermolysin Cyanogen Bromide
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Carnegie P R
References (38)
38 references, click to expand
  1. Digestion of an arg-pro bond by trypsin in the ecephalitogenic basic protein of human myelin.
    Nature. 1969 Aug 30;223(5209):958-9 PMID: 5803401
  2. Amino acid sequence adjoining the lone tryptophan of human serum albumin. A binding site of the protein.
    Biochemistry. 1970 Jun 23;9(13):2570-4 PMID: 5450224
  3. The identity of a myelin-like fraction isolated from developing brain.
    Biochem J. 1970 Dec;120(3):635-42 PMID: 5499976
  4. Experimental autoimmune encephalomyelitis. Digestion of basic protein of human myelin with cyanogen bromide and trypsin.
    Immunology. 1970 Jul;19(1):55-63 PMID: 4098596
  5. Encephalitogenic protein: structure.
    Science. 1969 May 2;164(3879):577-80 PMID: 5778011
  6. The action of trypsin on polylysine.
    Biochem J. 1953 Sep;55(2):328-37 PMID: 13093686
  7. The structure of the terminal regions of the encephalitogenic basic protein from bovine myelin.
    Arch Biochem Biophys. 1969 Dec;135(1):324-33 PMID: 4983100
  8. Role of a buried acid group in the mechanism of action of chymotrypsin.
    Nature. 1969 Jan 25;221(5178):337-40 PMID: 5764436
  9. Isolation and partial characterization of methylated arginines from the encephalitogenic basic protein of myelin.
    Biochem J. 1971 Jun;123(1):69-74 PMID: 5128665
  10. A simple procedure for the fractionation of the tryptic peptides of the C-terminal half of immunoglobulin lambda-chains.
    Biochem J. 1968 Dec;110(4):652-4 PMID: 4178701
  11. Strategy and tactics in protein chemistry.
    Biochem J. 1970 Oct;119(5):805-22 PMID: 4923920
  12. Physical properties of the bovine encephalitogenic protein; molecular weight and conformation.
    J Neurochem. 1970 Aug;17(8):1121-32 PMID: 5457621
  13. The isolation and properties of experimental allergic encephalitogenic protein.
    Biochem J. 1969 Mar;111(5):629-36 PMID: 4306464
  14. The use of thermolysin in amino acid sequence determination.
    Biochem J. 1968 Aug;108(5):893-5 PMID: 5673536
  15. Essential chemical requirements for induction of allergic encephalomyelitis.
    Nature. 1971 Jan 1;229(5279):22-4 PMID: 4098981
  16. Immunologic properties of the main encephalitogenic peptide from the basic protein of human myelin.
    J Immunol. 1970 Nov;105(5):1223-30 PMID: 4099924
  17. Allergic encephalomyelitis: the structure of encephalitogenic determinant.
    Proc Natl Acad Sci U S A. 1968 Oct;61(2):644-50 PMID: 4879399
  18. Lymphocyte sensitisation: an in-vitro test for cancer?
    Lancet. 1970 Dec 26;2(7687):1337-41 PMID: 4098910
  19. AN ENCEPHALITOGENIC PROTEIN OF HUMAN ORIGIN: SOME CHEMICAL AND BIOLOGICAL PROPERTIES.
    Ann N Y Acad Sci. 1965 Mar 31;122:182-98 PMID: 14313478
  20. Failure to induce myelin sheath formation around artificial fibres with a note on the toxicity of polyester fibres for nervous tissue in vitrol.
    J Neurol Sci. 1969 Jan-Feb;8(1):129-41 PMID: 5790365
  21. Human immunoglobulin subclasses. Partial amino acid sequence of the constant region of a gamma 4 chain.
    Biochem J. 1970 Mar;117(1):33-47 PMID: 4192699
  22. Electrophoretic mobilities of peptides on paper and their use in the determination of amide groups.
    Nature. 1966 Aug 6;211(5049):591-3 PMID: 5968723
  23. Localization of encephalitogenic basic protein in the intraperiod line of lamellar myelin.
    Nature. 1970 Sep 12;227(5263):1133-4 PMID: 5451106
  24. A COMPOSITIONAL ASSAY FOR INSULIN APPLIED TO A SEARCH FOR "PROINSULIN".
    J Biol Chem. 1965 Apr;240:1619-25 PMID: 14285500
  25. Properties, structure and possible neuroreceptor role of the encephalitogenic protein of human brain.
    Nature. 1971 Jan 1;229(5279):25-8 PMID: 4098982
  26. Specific enzymic methylation of an arginine in the experimental allergic encephalomyelitis protein from human myelin.
    Science. 1971 Feb 12;171(3971):579-81 PMID: 4924231
  27. Encephalitogenic basic protein from sciatic nerve.
    FEBS Lett. 1969 Jul;4(1):9-12 PMID: 11947131
  28. Glial cells and the central myelin sheath.
    Physiol Rev. 1968 Jan;48(1):197-251 PMID: 4866614
  29. Fractionation of encephalitogenic polypeptides from bovine spinal cord by gel filtration in phenol--acetic acid--water.
    Immunology. 1967 Feb;12(2):133-45 PMID: 6020118
  30. The maturation of human white matter myelin. Fractionation of the myelin membrane proteins.
    Biochemistry. 1968 Dec;7(12):4455-65 PMID: 5700665
  31. Myelin basic proteins of the rat central nervous system. Purification, encephalitogenic properties, and amino acid compositions.
    Biochim Biophys Acta. 1970 Feb 17;200(2):353-62 PMID: 5416126
  32. Allergic encephalomyelitis: a comparison of the encephalitogenic A1 protein from human and bovine brain.
    Arch Biochem Biophys. 1970 Jun;138(2):606-13 PMID: 5433591
  33. Chemical studies on experimental allergic encephalomyelitis. Peptide as the common denominator in all encephalitogenic 'antigens'.
    J Neurochem. 1966 Mar;13(3):127-62 PMID: 5939988
  34. Anomalous cleavage of aspartyl-proline peptide bonds during amino acid sequence determinations.
    Biochem Biophys Res Commun. 1970 Sep 10;40(5):1173-8 PMID: 4100801
  35. N-terminal sequence of an encephalitogenic protein form human myelin.
    Biochem J. 1969 Jan;111(2):240-2 PMID: 5763789
  36. THE AMINO ACID SEQUENCE OF PSEUDOMONAS CYTOCHROME C-551.
    Biochem J. 1963 Nov;89:349-78 PMID: 14084622
  37. Experimental allergic encephalomyelitis. Isolation of basic proteins and polypeptides from central nervous tissue.
    Biochem J. 1967 Nov;105(2):559-68 PMID: 5583998
  38. Histochemistry of myelin. XI. Loss of basic protein in early myelin breakdown and multiple sclerosis plaques.
    Histochem J. 1970 Jul;2(4):323-8 PMID: 4112999
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1230057
Published
1971-06-00
Pages
57-67
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1176899
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1230057 PMC Full Text View journal details
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