Inactivation of polykaryocytogenic and hemolytic activities of Sendai virus by phospholipase B (lysolecithinase).

Journal of virology ·Vol. 8 ·No. 5 ·1971-11-00 ·Pages 796-800

Barbanti-Brodano G, Possati L, La Placa M

Abstract

Evidence is presented that lysolecithin is involved in the fusing and hemolytic activities of Sendai virus. Treatment of the virus with phospholipase B (lysolecithinase) specifically inactivates the hemolytic and fusing abilities, without affecting the infectivity and the capability of the virus to hemagglutinate and adsorb to cells. The possible identity of lysolecithin with the "cell fusion factor" of paramyxoviruses and the mechanism of cell fusion are discussed.

MeSH Terms

Adsorption Animals Carcinoma Cell Fusion Cell Line Chick Embryo Cytopathogenic Effect, Viral Erythrocytes/immunology Guinea Pigs Hemagglutination Hemagglutination Tests Hemolysis Humans Lysophosphatidylcholines/metabolism Microscopy, Electron Mouth Neoplasms Parainfluenza Virus 1, Human/drug effects,immunology,pathogenicity Phospholipases/metabolism,pharmacology Phosphotungstic Acid Staining and Labeling Virus Cultivation

Chemicals

Lysophosphatidylcholines Phosphotungstic Acid Phospholipases

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Barbanti-Brodano G

Possati L

La Placa M

References (8)

8 references, click to expand

The lipids of paramyxoviruses: a comparative study of Sendai and Newcastle disease viruses.
Virology. 1968 Oct;36(2):286-92 PMID: 4300882
Studies on Sendai virus cell fusion factor.
Proc Soc Exp Biol Med. 1968 Dec;129(3):854-7 PMID: 4302155
Metabolism of lecithin and virus-induced cell fusion.
Proc Soc Exp Biol Med. 1969 Mar;130(3):903-8 PMID: 4304351
POLYKARYOCYTOSIS INDUCED BY NEWCASTLE DISEASE VIRUS IN MONOLAYERS OF ANIMAL CELLS.
Virology. 1965 Jun;26:228-45 PMID: 14323991
Lysolecithin and cell fusion.
Nature. 1970 Aug 22;227(5260):810-4 PMID: 5432243
[The lipid nature of substances responsible for the hemolytic activity of Myxo-virus paraninfluenzae I (Sendai virus)].
Ann Inst Pasteur (Paris). 1962 Feb;102:137-52 PMID: 14490823
EFFECT OF TRYPSIN TREATMENT ON THE HAEMOLYTIC, HAEMAGGLUTINATING, NEURAMINIDASE AND ADENOSINE DIPHOSPHATASE ACTIVITIES OF SENDAI VIRUS.
Acta Virol. 1963 Nov;7:490-7 PMID: 14098063
Presence of 2 different viral agents in brain cells of patients with subacute sclerosing panencephalitis.
Proc Soc Exp Biol Med. 1970 May;134(1):230-6 PMID: 4316388

Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.8.5.796-800.1971

Published

1971-11-00

Pages

796-800

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC376262

Subset

External Links

PubMed source DOI: 10.1128/JVI.8.5.796-800.1971 PMC Full Text View journal details

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Inactivation of polykaryocytogenic and hemolytic activities of Sendai virus by phospholipase B (lysolecithinase).

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